The thylakoid membranes isolated from spinach chloroplasts were immobilized on a flat gold film and a gold film having 600 nm nanowells in the absence or presence of 20 nm gold colloids. The immobilized thylakoids showed photosynthetic properties upon surface plasmon (SP) enhanced excitation. The localization of SP field by the randomly distributed gold nanowells was confirmed by the specific light scattering and transmission properties. The thylakoids immobilized in/over the gold nanowells showed specific fluorescence spectrum suggesting the excitation of chlorophylls by the localized SP field in/around the nanowells.
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