The DLR Earth Sensing Imaging Spectrometer (DESIS) is a new space-based hyperspectral sensor developed and operated by a collaboration between the German Aerospace Center (DLR) and Teledyne Brown Engineering (TBE). DESIS will provide hyperspectral data in the visible to near-infrared range with high resolution and near-global coverage. TBE provides the platform and infrastructure for the operation on the International Space Station (ISS), DLR has developed the instrument. This paper gives an overview of the design of the DESIS instrument together with results from the optical on-ground calibration. In-flight calibration, stability of dark signal and rolling vs. global shutter analysis will be presented.