Mr. Avishay Bransky Profile

Avishay Bransky

at Technion-Israel Institute of Technology

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Publications (2)

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Proceedings Article | 14 December 2006 Paper

A microfabricated bio-sensor for erythrocytes deformability and volume distributions analysis

Avishay Bransky, Natanel Korin, Yael Nemirovski, Uri Dinnar

Proceedings Volume 6416, 64160O (2006) https://doi.org/10.1117/12.695562

KEYWORDS: Microfabrication, Image processing, Glasses, Particles, Image segmentation, Blood, Biosensors, Biological research, Diagnostics, Optical spheres

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The deformability of erythrocytes is of great importance for oxygen delivery in the microcirculation. Reduced RBC deformability is associated with several types of hemolytic anaemias, malaria, sepsis and diabetes. Aging of erythrocytes is also associated with loss of deformability as well as reduction in cell volume. An automated rheoscope has been developed, utilizing a microfabricated glass flow cell, high speed camera and advanced image-processing software. RBCs suspended in a high viscosity medium were filmed flowing through a microchannel. The system produces valuable data such as velocity profiles of RBCs, spatial distribution within the microchannel, cell volume and deformation index (DI) curves. The variation of DI across the channel height, due to change in shear stress, was measured for the first time. Such DI curves were obtained for normal and Thalassemia RBCs and their diagnostic potential was demonstrated. The spatial distribution and velocity of RBCs and rigid microspheres were measured. Both RBC and rigid spheres showed enhanced inward lateral migration, however the RBCs form a depletion region at the center of flow. The volume and surface area of the flowing cells have been estimated based on a fluid mechanics model and experimental results and fell within the normal range. Hence, the system developed, provides means for examining the behavior of individual RBCs in microchannels, and may serve as a microfabricated diagnostic device for deformability and volume measurements.

Proceedings Article | 14 December 2006 Paper

The culture of human embryonic stem cells in microchannel perfusion bioreactors

Natanel Korin, Avishay Bransky, Uri Dinnar, Shulamit Levenberg

Proceedings Volume 6416, 64160N (2006) https://doi.org/10.1117/12.695558

KEYWORDS: Oxygen, Stem cells, Semiconducting wafers, Microfluidics, Cell biology, Computer aided design, Diffusion, Biology, Hypoxia, Tissue engineering

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The culture of human Embryonic Stem (ES) cells in microchannel bioreactors can be highly beneficial for ES cell biology studies and ES tissue engineering applications. In the present study we examine the use of Human Foreskin Fibroblasts (HFF) cells as feeder cells for human ES culture in a microchannel perfusion bioreactor. PDMS microchannels (depth:130 micron) were fabricated using conventional soft-lithography techniques. The channels were sterilized, coated with a human fibronectin solution and seeded with cells. Following a period of static incubation, culture medium was perfused through the channels at various flow rates and cell growth was monitored throughout the culture process. Mass transport and fluid mechanics models were used to evaluate the culture conditions (shear stress, oxygen levels within the micro-bioreactor as a function of the medium flow rate. The conditions for successful long-term culture (>7 days) of HFF under flow were established. Experiments with human embryonic stem cells cultured in microchannels show that the conditions essential to co-culture human ES cell on HFF cells under perfusion differ from the conditions necessary for HFF cell culture. Human ES cells were found to be highly sensitive to flow and culture conditions and did not grow under flow rates which were suitable for HFF long-term culture. Successful culture of undifferentiated human ES cell colonies in a perfusion micro-bioreactor is a basic step towards utilizing microfluidic techniques to explore stem cell biology.

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