The precise location and nature of the artery water permeability barrier is unknown.
Water imaging in arteries with femtosecond coherent anti-Stokes Raman scattering microscopy
reveals that this barrier is formed by the endothelial basolateral membrane.
The design of modern imaging systems is intricately concerned with the control of optical aberrations in systems that can
be manufactured at acceptable cost and with acceptable manufacturing tolerances. Traditionally this involves a multi-parameter
optimisation of the lens optics to achieve acceptable image quality at the detector. There is increasing interest
in a more generalised approach whereby digital image processing is incorporated into the design process and the
performance metric to be optimised is quality of the image at the output of the image processor. This introduces the
possibility of manipulating the optical transfer function of the optics such that the overall sensitivity of the imaging
system to optical aberrations is reduced. Although these hybrid optical/digital techniques, sometimes referred as
wavefront coding, have on occasion been presented as a panacea, it is more realistic to consider them as an additional
parameter in the optimisation process. We will discuss the trade-offs involved in the application of wavefront coding to
low-cost imaging systems for use in the thermal infrared and visible imaging systems, showing how very useful
performance enhancements can be achieved in practical systems.