New super-resolution optical imaging methods are emerging, but there are still deficiencies in the experiments to verify these methods. The problem is that when we need to image and verify experimental samples, we do not know the Ground Truth of the samples. Therefore, we propose a method to generate experimental samples with known Ground Truth. For imaging optical methods, we introduce a super-resolution nanoprobe that can scan in two dimensions, and in the detection optical path of the actual wide-field experiment, we modify the point spread function of the system to a single helix point spread function. By using a series of single helix point spread function samples collected from the wide-field imaging experiment, combined with the scanning of the optical needle, we finally generate images scanned by the optical needle using this generation method. These images are composed of single helix point spread functions, and their Ground Truth is known. Therefore, it is proved that using our method to generate images of some samples captured by imaging systems with known Ground Truth is a feasible scheme for imaging technology verification.
Optical microscopy has been indispensable for physiologically visualizing neuronal structure and function, while it remains a challenge since the limited diffraction resolution and restricted imaging depth. Nonlinear multifocal structured illumination microscopy (MSIM) provides resolution-doubled images and good penetration, but its performance is seriously degraded in deep tissues. Thus, we present a non-inertial scanning nonlinear MSIM system combined with adaptive optics (AO) to realize super-resolution imaging with aberration correction in vivo. It is indispensable for visualizing various synaptic spines and detailed morphological changes of neurons in vivo. Using the nonlinear MSIM for SHG imaging, collagen fiber alignments were quantified in various tissues, demonstrating its feasibility for identifying collagen characteristics.
Super-resolution optical fluctuation imaging (SOFI) uses the advantages of computational post-processing to solve the super-resolution problem under high labeling density. Higher-order SOFI requires sufficient fluorescence fluctuation information to reduce artifacts in reconstructed images. However, the length of image sequence is limited for a certain kind of fluorophores, such as the inherent lifetime. In this research, we proposed a method to enhance image quality using double-color acquisitions and then coupling them into sequences. The theoretical basis and the working principle of this method will be explained. Simulations and limitations analysis between typical acquisition and this method will also be given. Simulations demonstrated that this method can improve the overall image quality by about 31.58 % in the 2nd order SOFI and 56.26 % in the 4th order SOFI.
KEYWORDS: Luminescence, Microscopy, Signal to noise ratio, Super resolution, Imaging systems, Molecules, 3D image processing, Objectives, Point spread functions, Biomedical optics
Recently, three-dimensional (3D) super resolution imaging of cellular structures in thick samples has been enabled with the wide-field super-resolution fluorescence microscopy based on double helix point spread function (DH-PSF). However, when the sample is Epi-illuminated, much background fluorescence from those excited molecules out-of-focus will reduce the signal-to-noise ratio (SNR) of the image in-focus. In this paper, we resort to a selective-plane illumination strategy, which has been used for tissue-level imaging and single molecule tracking, to eliminate out-of-focus background and to improve SNR and the localization accuracy of the standard DH-PSF super-resolution imaging in thick samples. We present a novel super-resolution microscopy that combine selective-plane illumination and DH-PSF. The setup utilizes a well-defined laser light sheet which theoretical thickness is 1.7μm (FWHM) at 640nm excitation wavelength. The image SNR of DH-PSF microscopy between selective-plane illumination and Epi-illumination are compared. As we expect, the SNR of the DH-PSF microscopy based selective-plane illumination is increased remarkably. So, 3D localization precision of DH-PSF would be improved significantly. We demonstrate its capabilities by studying 3D localizing of single fluorescent particles. These features will provide high thick samples compatibility for future biomedical applications.
X-ray phase-contrast imaging is an important diagnostic tool in medicine, biology and materials science. In-line hard x-ray phase-contrast imaging is based on Fresnel diffraction of x-ray, therefore we propose to make phase retrieval calculations between arbitrary planes interrelated through the Fresnel domain. A new approach to the numerical reconstruction of object phase by the diffraction intensity for in-line x-ray phase-contrast imaging is presented. The new method is tested on simulated image and the results demonstrate the validity of this new approach.
In order to implement 3-D imaging of objects with large height discontinuities and/or surface isolation, we present a novel 3-D imaging system based on temporal sequential fringe projector to provide multi-resolution 3-D reconstruction. To recover the range data of such a surface, an enhanced scheme for temporal phase unwrapping procedure is proposed. We also describe methods for extracting the color texture corresponding to a range image. Experimental results are given to illustrate the validity of our proposed method.
In the spectrum range of middle wave infrared region (MWIR) and the long wave infrared region (LWIR) radiation, the infrared spectral imaging technology is far from mature in comparison with its counterpart in visible region because infrared radiation is relative weak, the corresponding solid-state detectors and dispersive elements are extremely expensive. The paper reports a novel configuration that exploits the abundant chromatic aberration of binary optical lens to create a dual band infrared imaging imager. The design method of spectrum-dividing systems is presented for infrared imaging spectrometer. The system was analysed and evaluated by optical design software ZEMAX, theoretical formulas were then established. The practical design shows that the system has the very simple optical design that enables a very low cost lightweight robust dual band infrared imaging spectrometer.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.