Dr. Brian A. Herman Profile

Dr. Brian A. Herman

Vice President for Research at Univ of Texas Health Science Ctr at San Antonio

SPIE Involvement:

Author

Publications (16)

SPIE Journal Paper | 1 May 2008 Open Access

Nonlinear scaling analysis of glucose metabolism in normal and cancer cells

V. Krishnan Ramanujan, Brian Herman

JBO, Vol. 13, Issue 03, 031219, (May 2008) https://doi.org/10.1117/12.10.1117/1.2928154

KEYWORDS: Cancer, Glucose, Mode conditioning cables, Luminescence, In vivo imaging, Fluorescent proteins, Tumors, Imaging systems, Data acquisition, Nonlinear optics

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Proceedings Article | 14 February 2007 Paper

Fluorescence lifetime contrast in small animal imaging

V. Krishnan Ramanujan, Abhik Bandyopadhyay, Luzhe Sun, Brian Herman

Proceedings Volume 6449, 644911 (2007) https://doi.org/10.1117/12.701133

KEYWORDS: Tumors, Luminescence, Tissues, Fluorescence lifetime imaging, Mouse models, Imaging systems, Preclinical imaging, Tissue optics, Animal model studies, Fluorescent proteins

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Proceedings Article | 7 February 2007 Paper

Mitochondrial dysfunction modulates nonlinear dynamics in liver cell metabolism

V. Krishnan Ramanujan, Brian Herman

Proceedings Volume 6436, 64360G (2007) https://doi.org/10.1117/12.701089

KEYWORDS: Mode conditioning cables, Liver, Nonlinear dynamics, Statistical analysis, Modulation, Proteins, Data acquisition, Luminescence, Chemical analysis, Gait analysis

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Proceedings Article | 15 February 2006 Paper

Time-resolved nanoscale imaging of biomolecules in living cells and tissues: prospects for small-animal imaging

V. Krishnan Ramanujan, Joe Robert Mireles, Brian Herman

Proceedings Volume 6089, 608901 (2006) https://doi.org/10.1117/12.643956

KEYWORDS: Tissues, Tumors, Luminescence, Imaging systems, Signal to noise ratio, Optical imaging, Animal model studies, Solids, In vivo imaging, Green fluorescent protein

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SPIE Journal Paper | 1 September 2005 Open Access

Multiphoton fluorescence lifetime contrast in deep tissue imaging: prospects in redox imaging and disease diagnosis

Venkata Ramanujan, Jian-Hua Zhang, Eva Biener, Brian Herman

JBO, Vol. 10, Issue 05, 051407, (September 2005) https://doi.org/10.1117/12.10.1117/1.2098753

KEYWORDS: Tissues, Luminescence, Multiphoton fluorescence microscopy, Photons, Fluorescence lifetime imaging, Tumors, Imaging systems, Sensors, Green fluorescent protein, Flavoproteins

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Proceedings Article | 21 June 2004 Paper

Multiphoton FLIM: a reliable FRET detection tool in cell biological applications

Ramanujan Krishnan, Eva Biener, Victoria Centonze, Arieh Gertler, Brian Herman

Proceedings Volume 5323, (2004) https://doi.org/10.1117/12.528050

KEYWORDS: Fluorescence resonance energy transfer, Fluorescence lifetime imaging, Luminescence, Receptors, Microscopes, Single photon, Imaging systems, Image fusion, Multiphoton microscopy, Microscopy

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Proceedings Article | 10 July 2003 Paper

Multiphoton fluorescence lifetime imaging microscopy (FLIM) using a streak camera

Ramanujan Krishnan, Haruhisa Saitoh, Hirotoshi Terada, Victoria Centonze, Brian Herman

Proceedings Volume 4963, (2003) https://doi.org/10.1117/12.478001

KEYWORDS: Fluorescence lifetime imaging, Multiphoton fluorescence microscopy, Imaging systems, Streak cameras, Fluorescence resonance energy transfer, Calibration, Temporal resolution, Data acquisition, Luminescence, Multiphoton microscopy

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SPIE Journal Paper | 1 July 2003 Open Access

Quantitative imaging of protein–protein interactions by multiphoton fluorescence lifetime imaging microscopy using a streak camera

Ramanujan Krishnan, Atsushi Masuda, Victoria Centonze, Brian Herman

JBO, Vol. 8, Issue 03, (July 2003) https://doi.org/10.1117/12.10.1117/1.1577574

KEYWORDS: Fluorescence lifetime imaging, Proteins, Luminescence, Imaging systems, Fluorescence resonance energy transfer, Energy transfer, Streak cameras, Multiphoton fluorescence microscopy, Temporal resolution, Molecules

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Proceedings Article | 17 June 2002 Paper

FRET imaging microscopy

Brian Herman, Mao Sun, Atsushi Masuda, Hans Gerritsen, Victoria Centonze

Proceedings Volume 4620, (2002) https://doi.org/10.1117/12.470683

KEYWORDS: Fluorescence resonance energy transfer, Molecules, Luminescence, Cell death, Microscopy, Microscopes, Multiphoton microscopy, Energy transfer, Electronic filtering, Fluorescence lifetime imaging

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Proceedings Article | 24 April 2001 Paper

Observation of real-time interactions of Bcl-2 family members during apoptosis

Brian Herman, Victoria Frohlich, Ming Qiu, Akiyuki Takahashi

Proceedings Volume 4262, (2001) https://doi.org/10.1117/12.424549

KEYWORDS: Cell death, Fluorescence resonance energy transfer, Proteins, Data modeling, Luminescence, Image filtering, Molecules, Molecular interactions, Green fluorescent protein, Image fusion

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Proceedings Article | 10 May 1996 Paper

Fluorescence lifetime imaging in cell biology

Brian Herman, Xue Wang, Ammasi Periasamy, Seongwook Kwon, Gerald Gordon, Pawel Wodnicki

Proceedings Volume 2678, (1996) https://doi.org/10.1117/12.239553

KEYWORDS: Fluorescence lifetime imaging, Luminescence, Calcium, Environmental sensing, Image intensifiers, Microscopes, Cell biology, Microscopy, Signal detection, Molecules

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Proceedings Article | 12 April 1995 Paper

Highly sensitive detection of human papillomavirus type 16 DNA using time-resolved fluorescence microscopy and long lifetime probes

Xue Wang, Ammasi Periasamy, Pawel Wodnicki, M. Siadat-Pajouh, Brian Herman

Proceedings Volume 2387, (1995) https://doi.org/10.1117/12.206816

KEYWORDS: Luminescence, Microscopy, Time resolved spectroscopy, Image intensifiers, Microscopes, Fluorescence lifetime imaging, Tissues, Cervical cancer, Europium, Signal detection

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We have been interested in the role of Human Papillomavirus (HPV) in cervical cancer and its diagnosis; to that end we have been developing microscopic imaging and fluorescent in situ hybridization (FISH) techniques to genotype and quantitate the amount of HPV present at a single cell level in cervical PAP smears. However, we have found that low levels of HPV DNA are difficult to detect accurately because theoretically obtainable sensitivity is never achieved due to nonspecific autofluorescence, fixative induced fluorescence of cells and tissues, and autofluorescence of the optical components in the microscopic system. In addition, the absorption stains used for PAP smears are intensely autofluorescent. Autofluorescence is a rapidly decaying process with lifetimes in the range of 1-100 nsec, whereas phosphorescence and delayed fluorescence have lifetimes in the range of 1 microsecond(s) ec-10 msec. The ability to discriminate between specific fluorescence and autofluorescence in the time-domain has improved the sensitivity of diagnostic test such that they perform comparably to, or even more sensitive than radioisotopic assays. We have developed a novel time-resolved fluorescence microscope to improve the sensitivity of detection of specific molecules of interest in slide based specimens. This time-resolved fluorescence microscope is based on our recently developed fluorescence lifetime imaging microscopy (FILM) in conjunction with the use of long lifetime fluorescent labels. By using fluorescence in situ hybridization and the long lifetime probe (europium), we have demonstrated the utility of this technique for detection of HPV DNA in cervicovaginal cells. Our results indicate that the use of time-resolved fluorescence microscopy and long lifetime probes increases the sensitivity of detection by removing autofluorescence and will thus lead to improved early diagnosis of cervical cancer. Since the highly sensitive detection of DNA in clinical samples using fluorescence in situ hybridization image is useful for the diagnosis of many other type of diseases, the system we have developed should find numerous applications for the diagnosis of disease states.

Proceedings Article | 17 August 1994 Paper

Fluorescence lifetime imaging microscopy (FLIM) and its applications

Xue Wang, Ammasi Periasamy, Gerald Gordon, Pawel Wodnicki, Brian Herman

Proceedings Volume 2137, (1994) https://doi.org/10.1117/12.182779

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Confocal microscopy, Receptors, Microscopy, Signal detection, Modulation, Time resolved spectroscopy, Image intensifiers, Microscopes

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SPIE Journal Paper | 1 February 1993

Multiple microscopic techniques for the measurement of plasma membrane lipid structure during hypoxia

Xue Wang, John Lemasters, Brian Herman, Scot Kuo

OE, Vol. 32, Issue 02, (February 1993) https://doi.org/10.1117/12.10.1117/12.60738

KEYWORDS: Plasma, Injuries, Fluorescence resonance energy transfer, Hypoxia, Luminescence, Optical tweezers, Image filtering, Video microscopy, Cell death, Data modeling

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Proceedings Article | 26 June 1992 Paper

Application of 3-D digital deconvolution to optically sectioned images for improving the automatic analysis of fluorescent-labeled tumor specimens

Stephen Lockett, Kenneth Jacobson, Brian Herman

Proceedings Volume 1660, (1992) https://doi.org/10.1117/12.59548

KEYWORDS: 3D image processing, Point spread functions, Image segmentation, Detection and tracking algorithms, Breast, Image processing, Tumors, Microscopy, Confocal microscopy, CCD cameras

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