The advantages of using electric fields to manipulate and assemble samples are the ability to control the force exerted on
particles and the simplicity of fabrication. Electroosmosis, an electrokinetic effect from the interaction between ions in
the electrical double layer and the electric field, has less dependency on material property compared to dielectrophoresis.
The critical parameters in electroosmosis are electrical double layer, the strength of electrical field and the flow velocity
field. In an attempt to find the correlation among these parameters, a 3D electroosmosis chip is fabricated with different
collection chamber size. The chamber size varying from 100 to 1000 μm was defined via photolithography and the size
effect on the accumulation efficiency and collection pattern were studied by numerical simulations and experiments. The
amine-modified polystyrene fluorescent particles whose average size is 1μm were used for experiments. The results
show that the collection efficiency is a combined effect of the strength of the tangential electric field and the flow
velocity gradient. As the chamber size decreases, the strength of the tangential field decreases and then increases, but the
gradient of flow velocity intensifies. For small chamber size, the tangential electrical field induces greater flow velocity
and results in more high velocity region. This explains why the smaller chamber size has better collection efficiency than
the larger chamber size. For electroosmosis collection, the size of the chamber is a critical parameter for efficiency
consideration.
Carbon nanotubes have been extensively studied due to their unique property and potential application like field
emission. For successful implementation, it is essential to know the properties of carbon nanotubes. In this work, we
propose a simple method to estimate the electric property of multi walled carbon nanotubes (MWCNTs) by ac
dielectrophoresis. Dielectrophoresis is a phenomenon resulted from the inhomogeneous electric field and has been used
to sort out colloids with different dielectric properties. When applied ac dielectrophoresis, the movement of the colloids
depends on the polarizability of the colloids relative to the medium as well as the applied frequency. In certain
frequency, the direction of dielectrophoresis force will change and this crossover frequency is related to the electric
property of colloids. Since the crossover frequency is a function of the particle's dielectric property, as a result, if the
crossover frequency can be obtained, then the electric property of MWCNTs can be estimated. In a preliminary
experiment, NanoAmor MWCNTs (95+%, core diameter: 5-10 nm, outside diameter 20-40 nm, length 5-15 μm) mixed with alcohol, DI water and the surfactant was injected onto a dielectrophoresis microfluidic chip to measure the
crossover frequency. These MWCNTs were under negative dielectrophoresis (repelled from high electric field) for
frequencies over 12 KHz, and were under positive dielectrophoresis (attracted to the high electric field) for frequencies
under 1 KHz. These results were compared with the CM factor frequency spectrum with known electric properties. The
results show that for positive dielectrophoresis in low frequency and negative dielectrophoresis in high frequency is a
characteristic of conducting materials which indicates that these MWCNTs are conducting in nature. One application of
this technique is the characterization of electric property of SWCNT which is currently under investigation.
The collecting and sorting micro size particles by electric force is easy to integrate with other bioassays. There are many
forms of electric forces such as electrophoresis, dielectrophoresis and electroosmosis which can be used to manipulate
particles. In an attempt to understand the role of electroosmosis and dielectrophoresis in the collection of micro size
particles, a small device made of two parallel plates is used to study the particle movement under AC electric field. The
device is fabricated by a top electrode and a bottom electrode separated by a spacer. The top electrode is made from an
ITO glass where the bottom electrode is made of Corning 1737 glass sputtered with chromium. A dielectric layer is
fabricated by spin coating a thin photo-resist (0.5~1μm) on the bottom electrode and a spacer made of curing PDMS is
utilized to separate these electrodes. A 900μm × 900μm collecting chamber is fabricated on the bottom electrode via
photolithography. The amine-modified polystyrene fluorescent particles whose average size is 1 μm were used for
collection experiments. Different frequency and power were applied to generate the non-uniform electric field. It was
found that frequency is the critical factor for electroosmotic velocity. There seems to be an optimum frequency that leads
to largest particle velocity. The underlying mechanism is believed to the competing forces among dielectrophoresis and
electroosmosis. This device demonstrates that the electroosmosis force is suitable for collecting bio-particles in AC
electric field.
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