Laser scanning confoncal technology was used to study relationship between apoptosis and change of Ca2+ induced by
CSA (Capparis spinosa L. total alkaloid, CSA) on human heptocarcinoma cell HepG-2. Killing effect of CSA on
human heptocarcinoma cell HepG-2 was measured by MTT method, while morphological observation of HepG-2 cells
was completed by fluorescence microscope. Apoptosis induced by CSA on HepG-2 cells was measured by
flowcytometry. In addition, change of intracellular Ca2+ level of CSA on HepG-2 cells was observed by laser scanning
confocal microscope. As a result, CSA had obvious cytotoxicity on HepG-2 in a dose-dependent manner, and its IC50
was 162.4μg/ml. CSA could induce characteristic apoptosic morphology of HepG-2 cells, and apoptosis percentage was
significantly higher than control one. Migration of cells cycle from S phase to G2 phase had been blocked by CSA.
Concentration of Ca2+ in HepG-2 had been increased by CSA, which was positive correlation with drug dosage. CSA
had obvious effect of killing and inducing apoptosis on human heptocarcinoma cell HepG-2, and overload of Ca2+ might
be invovled in these events.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.