In this work, a very simple electrochemical HIV-1 protease biosensor useful for the development of an inexpensive lab-on-a- chip (LOC) device was constructed. The detection mechanism was designed to minimize the complexity either in the recognition receptor immobilization step or during the detection itself. The magnetic self-assembled monolayer of HIV-1 protease substrate peptide was able to detect as low as 10 pg/ml of the protease within 25 minutes with high specificity.
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