We developed a smartphone-based epifluorescence microscope for fresh tissue imaging. The smartphone microscope optics was optimally designed to achieve similar resolution (0.56 μm) and FOV (520 μm) as the bench 40x microscope, commonly used during the histopathologic analysis. Preliminary images obtained from an excised human pancreatic tissue stained with a rapid staining fluorescence dye (PARPi-FL) clearly visualized individual tumor cells.
The ray formalism is critical to understanding light propagation, yet current pedagogy relies on inadequate 2D representations. We present a system in which real light rays are visualized through an optical system by using a collimated laser bundle of light and a fog chamber. Implementation for remote and immersive access is enabled by leveraging a commercially available 3D viewer and gesture-based remote controlling of the tool via bi-directional communication over the Internet.