Dr. Christine Austin Profile

Dr. Christine Austin

at Icahn School of Medicine at Mount Sinai

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 4 March 2022 Presentation + Paper

Signal collection through water-immersion objectives improves Raman spectral quality from dental tissues

Piyush Kumar, Christine Austin

Proceedings Volume 11942, 1194202 (2022) https://doi.org/10.1117/12.2609798

KEYWORDS: Raman spectroscopy, Objectives, Tissues, Teeth, Signal to noise ratio, Biological research, Raman scattering

Read Abstract +

Raman spectroscopy (RS) has emerged as a method of choice for label-free, nondestructive, objective, and rapid analysis of biological samples. RS has found profound applications in dental research with studies on early caries detection, carbonate and phosphate contents, mineralization and demineralization patterns and stresssignatures in teeth samples. Raman dental spectral features are usually affected by fluorescence, especially archaeological samples, and are prone to thermal damage when laser power is increased for superior signal-to-noise ratio. This affects the quality of Raman dental maps, limiting their utility. This also warrants the need for exhaustive spectral preprocessing. One way to refine signal quality is through enhanced collection efficiency. In this study, we explored if spectra-quality from dental samples can be improved by signal collection through water-immersion objectives, since informative Raman regions are not affected by water. Raman maps were obtained from tooth samples under air-immersion objectives (10X and 50X) and a water-immersion objective (63X) using 532 nm laser. Spectra acquired through the different objectives were compared. We observed that the water-immersion objective reduced fluorescence, decreased baseline, and prevented thermal heating/damage to the samples. Dental samples were not affected by prolonged immersion in water. The improved spectral quality allows lower acquisition-time for mapping experiments. Further, it also reduces the preprocessing required to obtain sufficient signal-to-noise quality. Thus, application of water-immersion objectives in Raman experiments has the potential to enhance spectral quality and experimental outcomes while keeping the samples preserved for further analyses.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years