The tympanic membrane (TM) is an important hearing component that when ruptured causes severe hearing difficulty. It has been mainly characterized mechanically by measuring its displacements, vibration modes, impedance, and thickness. The objective of this research is to determine the relationship of the TM’s thickness obtained with confocal laser scanning microscopy (with scans along the Z axis) with the magnitude of the TM’s surface displacements measured with sound stimuli of 1.8, 5.2, and 12 kHz using digital holographic interferometry. In order to correlate the data, three regions of four healthy cats’ TMs are studied, with a particular finding that the thickness is not the same in these regions and, among samples, a feature readily noticed in the magnitudes of the displacements. Through the relationship of the data from the TM’s surface displacements with its thickness, it is now possible to confidently detect pathological changes in its structure by simply quantifying the magnitude of the former, a characteristic corroborated by the Pearson correlation coefficient (r).
In optical metrology, non-destructive methods allow studying some mechanical properties of the samples to investigate by using light, which leads to non-contact testing. This paper shows recent results of the application of non-destructive optical methods based on Digital Holographic Interferometry to the study biological tissues; particularly vocal folds and the tympanic membrane. The displacements data and its corresponding patterns found generates information on its characteristics that can be correlated with their physiological state. These methods prove to be an alternative viable and appropriate to characterize these soft tissues so important for the proper function of the human body. The result shows a potential impact on its possible uses in the field of otorhinolaryngology.
A polarimetric characterization, consisting of the Mueller matrix determination and the
measurement of the refractive index, is employed to study bee honey and corn syrup differences.
Two samples of commercial marks of bee honey and one sample of commercial mark corn syrup
are studied. Results show the corn syrup and one of the bee honey samples have a similar
polarimetric behavior, which differs from the second bee honey sample. This behavior can be
employed as a simple, qualitative test, to discriminate true bee honey from corn syrup or from
adulterated bee honey.s-powe