Dr. Domnik Lenz Profile

Dr. Domnik Lenz

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Publications (15)

Proceedings Article | 29 February 2008 Paper

Immunological changes following protein losing enteropathy after surgery total cavopulmonary connection (TCPC) by cytomics

József Bocsi, Dominik Lenz, Anja Mittag, Ursula Sauer, Lena Wild, John Hess, Dietmar Schranz, Jörg Hambsch, Peter Schneider, Attila Tárnok

Proc. SPIE. 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI

KEYWORDS: Surgery, Proteins, Blood, Heart, Luminescence, Cardiology, Receptors, Analytical research, Biological research, Cardiovascular surgery

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Proceedings Article | 19 February 2007 Paper

Application of quantitative morphological cytometry for evaluation of shear stress: potential for HCS systems

Bartek Rajwa, Dominik Lenz, Bülent Bayraktar, Silas Leavesley, J. Paul Robinson

Proc. SPIE. 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V

KEYWORDS: Cytoskeletons, Image segmentation, Shape analysis, Classification systems, Image analysis, Image filtering, Blood circulation, Binary data, Microscopy, Image classification

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Proceedings Article | 27 March 2006 Paper

Automated four color CD4/CD8 analysis of leukocytes by scanning fluorescence microscopy using Quantum dots

Jozsef Bocsi, Anja Mittag, Viktor Varga, Bela Molnar, Zsolt Tulassay, Ulrich Sack, Dominik Lenz, Attila Tarnok

Proc. SPIE. 6096, Colloidal Quantum Dots for Biomedical Applications

KEYWORDS: Luminescence, Atomic force microscopy, Quantum dots, Microscopes, Blood, Biological research, Microscopy, Optical filters, Multiplexing, Analytical research

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Proceedings Article | 21 February 2006 Paper

Clinical cytomics

Attila Tárnok, Anja Mittag, Dominik Lenz

Proc. SPIE. 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV

KEYWORDS: Medicine, Tissues, Biological research, Proteins, Data acquisition, Surgery, Flow cytometry, Cancer, Heart, Multiplexing

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Proceedings Article | 1 April 2005 Paper

Small and cheap: accurate differential blood count with minimal sample volume by laser scanning cytometry (LSC)

Anja Mittag, Dominik Lenz, Paul Smith, Susanne Pach, Attila Tarnok

Proc. SPIE. 5692, Advanced Biomedical and Clinical Diagnostic Systems III

KEYWORDS: Blood, Luminescence, Diagnostics, Laser scanners, Statistical analysis, Argon ion lasers, Cardiology, Heart, Microscopes, Signal detection

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Aim: In patients, e.g. with congenital heart diseases, a differential blood count is needed for diagnosis. To this end by standard automatic analyzers 500 μl of blood is required from the patients. In case of newborns and infants this is a substantial volume, especially after operations associated with blood loss. Therefore, aim of this study was to develop a method to determine a differential blood picture with a substantially reduced specimen volume. Methods: To generate a differential blood picture 10 μl EDTA blood were mixed with 10 μl of a DRAQ5 solution (500μM, Biostatus) and 10 μl of an antibody mixture (CD45-FITC, CD14-PE, diluted with PBS). 20 μl of this cell suspension was filled into a Neubauer counting chamber. Due to the defined volume of the chamber it is possible to determine the cell count per volume. The trigger for leukocyte counting was set on DRAQ5 signal in order to be able to distinguish nucleated white blood cells from erythrocytes. Different leukocyte subsets could be distinguished due to the used fluorescence labeled antibodies. For erythrocyte counting cell suspension was diluted another 150 times. 20 μl of this dilution was analyzed in a microchamber by LSC with trigger set on forward scatter signal. Results: This method allows a substantial decrease of blood sample volume for generation of a differential blood picture (10 μl instead of 500μl). There was a high correlation between our method and the results of routine laboratory (r²=0.96, p<0.0001; n=40). For all parameters intra-assay variance was less than 7 %. Conclusions: In patients with low blood volume such as neonates and in critically ill infants every effort has to be taken to reduce the blood volume needed for diagnostics. With this method only 2% of standard sample volume is needed to generate a differential blood picture. Costs are below that of routine laboratory. We suggest this method to be established in paediatric cardiology for routine diagnostics and for resource poor settings.

Proceedings Article | 29 March 2005 Paper

Immunophenotyping by slide-based cytometry and by flow cytometry are comparable

Andreas Gerstner, Wiebke Laffers, Anja Mittag, Ingo Daehnert, Domnik Lenz, Friedrich Bootz, Jozsef Bocsi, Attila Tarnok

Proc. SPIE. 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III

KEYWORDS: Luminescence, Flow cytometry, Blood, Glasses, Laser scanners, Statistical analysis, Signal detection, Cardiology, Laser systems engineering, Monoclonal antibodies

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Proceedings Article | 24 March 2005 Paper

Tissomics: two- and three-dimensional distribution of nuclei in brain tissue using laser scanning cytometry (LSC)

Domnik Lenz, Anja Mittag, Birgit Mosch, Jozsef Bocsi, Thomas Arendt, Attila Tarnok

Proc. SPIE. 5701, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XII

KEYWORDS: Tissues, Brain, Luminescence, Solids, Neurons, Laser tissue interaction, Neuroimaging, Laser scanners, Argon ion lasers, Data acquisition

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Proceedings Article | 1 July 2004 Paper

Assessment of DNA replication in central nervous system by Laser Scanning Cytometry

Dominik Lenz, Birgit Mosch, Jozsef Bocsi, Thomas Arendt, Attila Tárnok

Proc. SPIE. 5322, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II

KEYWORDS: Neurons, Brain, Alzheimer's disease, Luminescence, Tissues, Laser scanners, Proteins, Data acquisition, Solids, Argon ion lasers

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Proceedings Article | 1 July 2004 Paper

Sequential photobleaching for increasing the measurable fluorochromes by laser scanning cytometry

Anja Mittag, Dominik Lenz, Jozsef Bocsi, Ulrich Sack, Andreas Gerstner, Attila Tarnok

Proc. SPIE. 5322, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II

KEYWORDS: Luminescence, Optical filters, Blood, Laser scanners, Statistical analysis, Data acquisition, Glasses, Biological research, Fluorescence resonance energy transfer, Microscopes

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For immunophenotypic analysis more measurable parameters for the discrimination of leukocyte subsets are necessary. With a single scan six fluorochromes can be distinguished with the Laser Scanning Cytometer (LSC). Due to the number of PMTs the amount of simultaneously measurable fluorescences per scan is limited. Nevertheless, the amount of measurable colors can be improved to eight by appropriate change of the filter settings and two scans per specimen. Aim of this study was to use the special features of Slide based Cytometry (SBC) beyond filter change, remeasurement and merging to distinguish fluorochromes with similar emission spectra. The photosensitivity of fluorochromes that are excited and emit in a similar wavelength range may be very different. The number of measurable parameters per PMT was increased using photosensitivity of different fluorochromes as additional criteria. Peripheral blood leukocytes were stained with antibodies conjugated to the fluorochromes APC, APC-Cy5.5 and Alexa-Fluor 633 and mounted on conventional uncoated glass slides with Fluorescence mounting medium. Specimens were excited in the LSC with the HeNe (633nm) Laser and measured at different filter settings (670/20nm-filter for APC/ALEXA 633 and 710/20nm-filter for APC-Cy5.5). At this point, APC-Cy5.5 and APC/ALEXA633 were already distinguishable. In order to differentiate between APC and ALEXA633 photobleaching was performed by repeated excitation with the laser at 633nm. Control measurements proved that APC is much more sensitive against laser excitation, i.e. looses much more fluorescence intensity than ALEXA633. The separate measurements (before/after filter change and before/after bleaching) were merged into one file. The photostability of Alexa-Fluor 633 (1.02% bleach per scan) and APC (5.74% bleach per scan) are substantially different. Therefore, after bleaching and merging both fluorochromes can be distinguished and are regarded by the software as separate parameters. The fluorochromes APC/ALEXA633 and APC-Cy5.5 can be discriminated by changing the emission filters before bleach. By sequential photobleaching, change of filters and subsequent merging of the data the number of simultaneously measurable “colors” is substantially increased.

Proceedings Article | 22 June 2004 Paper

Clinical and laboratory applications of slide-based cytometry with the LSC, SFM, and the iCYTE imaging cytometer instruments

Jozsef Bocsi, Ed Luther, Anja Mittag, Ingo Jensen, Ulrich Sack, Dominik Lenz, Lajos Trezl, Viktor Varga, Beea Molnar, Attila Tarnok

Proc. SPIE. 5328, Microarrays and Combinatorial Techniques: Design, Fabrication, and Analysis II

KEYWORDS: Atomic force microscopy, Cell death, Luminescence, Microscopes, Microscopy, Tumors, Digital imaging, Optical filters, Signal detection, Blood

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Proceedings Article | 19 June 2003 Paper

Six and more color immunophenotyping on the slide by laser scanning cytometry (LSC)

Dominik Lenz, Andreas Gerstner, Wiebke Laffers, Michae Steinbrecher, Friedrich Bootz, Attila Tarnok

Proc. SPIE. 4962, Manipulation and Analysis of Biomolecules, Cells, and Tissues

KEYWORDS: Optical filters, Luminescence, Bandpass filters, Blood, Laser scanners, Microscopes, Flow cytometry, Electronic filtering, Statistical analysis, Fluorescence correlation spectroscopy

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Proceedings Article | 19 June 2003 Paper

Detection of endothelial progenitor cells by flow and laser-scanning cytometry

Dominik Lenz, Karstem Lenk, Volker Adams, Andreas Boldt, Rainer Hambrecht, Attila Tarnok

Proc. SPIE. 4962, Manipulation and Analysis of Biomolecules, Cells, and Tissues

KEYWORDS: Blood, Luminescence, Laser scanners, Signal detection, Flow cytometry, Microscopes, Data acquisition, Light scattering, Visualization, Tissues

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Proceedings Article | 28 May 2002 Paper

Multicolor immunophenotyping of tissue sections by laser scanning cytometry (LSC)

Attila Tarnok, Andreas Gerstner, Dominik Lenz, Pavel Osmancik, Peter Schneider, Christine Trumpfheller, Pal Racz, Klara Tenner-Racz

Proc. SPIE. 4622, Optical Diagnostics of Living Cells V

KEYWORDS: Tissues, Luminescence, Lymphatic system, Data acquisition, Laser scanners, Argon ion lasers, Flow cytometry, Quantitative analysis, Microscopes, Laser tissue interaction

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Proceedings Article | 28 May 2002 Paper

Quantification of chemotaxis during pediatric cardiac surgery by flow and laser scanning cytometry

Attila Tarnok, Joerg Schmid, Pavel Osmancik, Dominik Lenz, Michal Pipek, Joerg Hambsch, Andreas Gerstner, Peter Schneider

Proc. SPIE. 4622, Optical Diagnostics of Living Cells V

KEYWORDS: Surgery, Blood, Laser scanners, Optical filters, Flow cytometry, Luminescence, Laser therapeutics, Microscopes, Monoclonal antibodies, In vitro testing

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Proceedings Article | 28 May 2002 Paper

Novel approaches for immunophenotyping by laser scanning cytometry (LSC)

Andreas Gerstner, Wiebke Laffers, Friedrich Bootz, Dominik Lenz, Attila Tarnok

Proc. SPIE. 4622, Optical Diagnostics of Living Cells V

KEYWORDS: Luminescence, Laser scanners, Microscopes, Blood, Flow cytometry, Optical filters, Statistical analysis, Laser therapeutics, Glasses, Photomicroscopy

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