Micro X-ray Fluorescence (MXRF) has proven to be a powerful tool in the rapid and quantitative means of screening oliogpeptides. MXRF is a non-destructive method of analysis, which can detect elemental composition of a sample by measuring its characteristic X-ray emission wavelengths or energies. An effective high throughput screening technique is described for the rapid screening of bead-based libraries by MXRF in order to identify suitable chelating agents that will bind metals found in radioactive dispersive devices. It is a sensitive technique which in conjunction with the wide range of chemistry inherent in peptide libraries (e.g. varying charge, length, hydrophobicity, aromaticity etc.), provides a rapid and quantitative means for screening chelator-ion binding. The method involves the selection of a suitable library of ligands; in this case it is a bead-based library of peptides. The library is exposed to the cation of interest and immobilized on to a microarray. The array is then analyzed by MXRF enabling rapid identification of chelating agents. This enables the screening of approximately 27,500 sequences per day. Initial experiments carried out successfully identified sequences that are selective for Co under certain binding conditions. This involved the screening of 8,400 sequences in adverse environmental conditions containing possible interferences (e.g. Ca, Fe, Al, Cs, Ir), which could be encountered in our application.
An effective high throughput screening technique is described for the rapid analysis of solid-supported libraries to identify selective chelating agents for metals, focusing on those possibly present in radioactive dispersal devices. Micro X-ray Fluorescence (MXRF) is the method of choice. MXRF is a rapid and sensitive technique which allows the measurement of metal-chelator complexes without significant sample preparation. For this study we have employed commercial peptide libraries and have examined factors such as side-chain charge, oligopeptide length, hydrophobicity, and aromaticity in relation to metal binding. MXRF provides a rapid and quantitative means for screening chelator-ion binding. Initial experiments carried out successfully identified sequences that are selective for Co under certain binding conditions containing possible interferences (e.g. Ca, Fe, Al, Cs, Ir), which could be encountered in our application.
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