Prof. Eizo Watanabe Profile

Prof. Eizo Watanabe

at Chiba Univ.

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Publications (2)

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Proceedings Article | 5 March 2021 Presentation + Paper

Motion picture acquisition and analysis of microcirculation in septic model rats

Mami Kawasaki, Kazuya Nakano, Takashi Ohnishi, Masashi Sekine, Eizo Watanabe, Shigeto Oda, Taka-aki Nakada, Hideaki Haneishi

Proceedings Volume 11631, 116310S (2021) https://doi.org/10.1117/12.2581275

KEYWORDS: Motion models, Motion analysis, Blood, Pathogens, Oxygen, Motion estimation, Analytical research

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Microcirculation plays an important role by supplying oxygen and nutrients to maintain our lives. It is known that, as pathogenesis of sepsis, the microcirculation is impaired at the early stage of sepsis. In this case, it is expected that thrombomodulin alfa (TM alfa) works for the recovery of microcirculation. Direct observation of the impairment and recovery of microcirculation related to sepsis may give us clear understanding of those phenomena. In this study, we conducted experiments to observe microcirculation of septic model rats with an optical system. In these experiments, by attaching a chamber to the back of each rat, the same region of the microcirculation was consecutively monitored. Then, these rats were divided into three groups: control model rats (sham group), septic model rats (diseased group), and septic model rats to which TM alfa was administered (treated group). Each group includes five rats. In the diseased group smaller vessels disappeared and larger vessels became thinner. On the other hand, the treated group showed at first the degradation of microcirculation then the recovery. This may indicate effectiveness of TM alfa. We also estimated the blood velocity and blood vessel diameter from the acquired motion pictures to evaluate condition of the microcirculation. As a result, we quantitatively confirmed while blood velocity and blood vessel diameter of the diseased group decreased, that of the treated group decreased and then recovered. It should be noted that the set of motion pictures obtained in these experiments has potentially useful information for further analysis and is to be open to relevant researchers.

Proceedings Article | 27 March 2019 Paper

Improvement of optical setup for microcirculation imaging and flow analysis in septic shock rats

Mami Kawasaki, Ryohei Hashimoto, Kazuya Nakano, Takashi Ohnishi, Masashi Sekine, Eizo Watanabe, Shigeto Oda, Hideaki Haneishi

Proceedings Volume 11050, 1105009 (2019) https://doi.org/10.1117/12.2521514

KEYWORDS: Blood, Blood vessels, Microcirculation imaging, Hemodynamics

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Microcirculation plays an important role in maintaining our lives. Observing the microcirculation has been considered important in understanding the disease mechanisms and diagnosing diseases. Sidestream dark-field (SDF) imaging is one of the methods to observe the microcirculation. However, the SDF imaging has several problems for instance artifacts caused by pressure and heat. Measurement points is under pressure because SDF imaging requires direct contact with measurement points, which may affect hemodynamics. Therefore, we construct a non-contact setup. Furthermore, at the early stage of sepsis, it is known that the microcirculation is impaired. To investigate the relationship between the flow of red blood cells (RBCs) and septic shock, we conducted an experiment using the setup to observe septic model rats and sham rats. Moreover, we calculated the blood velocity to estimate the flow of RBCs by using acquired motion pictures. We confirmed that the sham rats showed slight change in lactate value during the observation and improved the blood velocity compared with just after abdominal closure. However, lactate value of the septic model rats increased and the blood velocity of septic model rats decreased. This finding suggests that microcirculatory alteration may be a sign of sepsis and septic shock progression.

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