We present a computational modeling approach for imitation of the time-domain optical coherence tomography (OCT) images of biotissues. The developed modeling technique is based on the implementation of the Leontovich–Fock equation into the wave Monte Carlo (MC) method. We discuss the benefits of the developed computational model in comparison to the conventional MC method based on the modeling of OCT images of a nevus. The developed model takes into account diffraction on bulk-absorbing microstructures and allows consideration of the influence of the amplitude–phase profile of the wave beam on the quality of the OCT images. The selection of optical parameters of modeling medium, used for simulation of optical radiation propagation in biotissues, is based on the results obtained experimentally by OCT. The developed computational model can be used for imitation of the light waves propagation both in time-domain and spectral-domain OCT approaches.
We applied the method of statistical trials to the parabolic equation of laser radiation propagation in biotissue to perform a new method of optical coherence tomography modeling. Results of modeling tests show the efficiency of the developed approach.
Methods of two-photon microscopy are widely used in the study of biological objects, in particular, skin, due to the possibility to study objects both on the surface and at depth without attracting additional fluorophores due to endogenous autofluorescence. In this paper, the methods of image analysis of the AF signal and SHG signal are applied to assess the condition of the skin during the development of lymphedema. It is shown that for groups of healthy tissue and lymphedematous using SAAID distribution histograms, changes in tissues can be detected.
The results of a comparative study of an emanation from the skin of patients with diabetes mellitus and healthy volunteers using THz spectroscopy. A technique for collecting and analyzing emanation samples from the skin has been developed, and its applicability in THz spectroscopy has been demonstrated. Good separation of THz spectra of the skin emanations has been shown for the target and control groups by the principal component analysis. It shows that THz spectroscopy of skin emanations is useful for the diagnosis of diabetes mellitus.
The method of laser IR radiation propagation simulation in a case of randomly inhomogeneous media based on Leontovich – Fock equation in the application of optical coherent tomography modeling in biotissues is proposed. We describe the proposed methodology and demonstrate its implementation on a test case.
The changes in the stiffness modulus of collagen structures in a collagen phantom were studied using optical coherent elastography (OCE). The disorganization of the phantom collagen fibers was obtained by mechanical action (twisting). Young's modulus values were measured for various phantom density values using the compression OCE method.
The of multiphoton microscopy (MPM) based on two-photon fluorescence and second-harmonic generation is promising for diagnosing neoplastic transformation of tissues. The results of MPM studies of tissues with prostate adenocarcinoma embedded in paraffin blocks are presented. The tissue fluorescence was also analyzed using the fluorescence lifetime (Fluorescence-lifetime imaging microscopy - FLIM) option. The relationships between the fluorescence peculiarities and the second harmonic generation of the prostate gland tissues were obtained for different morphological images.
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