The viscoelastic properties and microstructure of the fibrin clot network are compromised in patients with coagulation abnormalities. In this study, we harness two novel optical approaches, iCoagLab and Spectrally Encoded Confocal Microscopy (SECM), to investigate how modifications in viscoelastic properties of blood clots are reflected in the microstructural features of the fibrin network in coagulopathic patients. We observe that blood clot viscoelasticity is directly related to fibrin density, fiber length, and fiber straightness of a fully polymerized fibrin network.
Rapidly changing coagulation status is a major challenge in managing and preventing bleeding in patients on mechanical circulatory support. Here, we apply iCoagLab, a hand-held coagulation sensing instrument for timely and comprehensive blood coagulation assessment in patients undergoing cardiac pump implantation. Our results confirm the high accuracy and precision of iCoagLab tests in quantifying key clotting parameters including clotting time, clot stiffness and clotting rate. These studies will help pave the way towards addressing bleeding complications at the point of treatment to potentially manage and prevent hemorrhagic events during mechanical circulatory support.
The complete blood count (CBC) is a foundational diagnostic test, but its accessibility is limited due to the blood draw, expensive laboratory equipment, and trained personnel required. Here, we present a cell phone microscope design for achieving phase contrast in high resolution capillary imaging, which allows individual blood cells to be imaged for a non-invasive CBC. The cell phone microscope uses a reversed lens as an objective to maintain a high resolution. Relay lenses create space for incorporation of an offset LED that can be critically imaged to produce oblique back illumination, resulting in phase contrast.
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