Felix Koberling received his Ph.D. in Physical Chemistry in 2001 from the University in Mainz, Germany working on single quantum dot microscopy. In 2001 he joined PicoQuant GmbH, one of the leading companies in time-resolved single photon counting where he is today Head of System Development. He developed the first commercially available confocal microscope with full fluorescence lifetime analysis capability down to the single molecule level. Furthermore, he worked on various single molecule and fluorescence analysis schemes like FLIM, PIE-FRET, time-gated FCS, FLCS and 2fFCS to establish them as standard techniques in biological and biophysical research.
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pH and chloride recordings in living cells using two-photon fluorescence lifetime imaging microscopy
Advanced FRET and FCS measurements with laser scanning microscopes based on time-resolved techniques
This will count as one of your downloads.
You will have access to both the presentation and article (if available).
This course introduces the basics of single molecule fluorescence spectroscopy and imaging under am-bient conditions especially for biologically relevant applications. The first part covers the technological requirements for single molecule fluorescence detection and spectroscopy, covering excitation sources, microscopy optics, optical and mechanical components, single photon detectors, fast photon-counting electronics, data acquisition, processing, visualization and evaluation.
The second part focuses on several single-molecule spectroscopic and imaging techniques: fluores-cence-correlation spectroscopy (FCS) and its different variants such as 2-focus FCS, fluorescence life-time correlations spectroscopy (FLCS), fluorescence cross-correlation spectroscopy (FCCS), or photo-electron transfer mediated FCS (PET-FCS); fluorescence intensity distribution analysis (FIDA) alias photon-counting histogram analysis (PCH); single-molecule Förster resonance energy transfer (smFRET); focused and defocused single-molecule imaging and fluorescence lifetime imaging (FLIM).
Selected applications will be presented: monitoring conformational changes in proteins, fast conforma-tional fluctuations in peptides, single-molecule motion and reorientation of polymer-embedded chro-mophores and of motor proteins, single molecule and single quantum dot photophysics, and super-resolution fluorescence microscopy.
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