Dr. Felix Koberling
Head of Systems Division at PicoQuant GmbH
SPIE Involvement:
Conference Chair | Editor | Author | Instructor
Area of Expertise:
research and development , single molecule detection , time-correlated single photon counting , fluorescence lifetime imaging , fluorescence correlation spectroscopy , superresolution microscopy
Websites:
Profile Summary

Felix Koberling received his Ph.D. in Physical Chemistry in 2001 from the University in Mainz, Germany working on single quantum dot microscopy. In 2001 he joined PicoQuant GmbH, one of the leading companies in time-resolved single photon counting where he is today Head of System Development. He developed the first commer­cially available confocal microscope with full fluorescence lifetime analysis capability down to the single molecule level. Furthermore, he worked on various single molecule and fluorescence analysis schemes like FLIM, PIE-FRET, time-gated FCS, FLCS and 2fFCS to establish them as standard techniques in biological and biophysical research.
Publications (37)

Proceedings Article | 5 March 2021 Presentation
Proc. SPIE. 11648, Multiphoton Microscopy in the Biomedical Sciences XXI
KEYWORDS: Fluorescence lifetime imaging, Visualization, Ultrafast imaging, Image resolution, Temporal resolution, Sensors, Picosecond phenomena, Fluorescence resonance energy transfer, Tissues, Single photon

Proceedings Article | 5 March 2021 Presentation
Proc. SPIE. 11650, Single Molecule Spectroscopy and Superresolution Imaging XIV

Proceedings Article | 5 March 2021 Presentation
Proc. SPIE. 11654, High-Speed Biomedical Imaging and Spectroscopy VI
KEYWORDS: Fluorescence lifetime imaging, Visualization, Ultrafast imaging, Image resolution, Temporal resolution, Sensors, Picosecond phenomena, Fluorescence resonance energy transfer, Tissues, Single photon

Proceedings Article | 26 March 2020 Presentation
Proc. SPIE. 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII
KEYWORDS: Fluorescence correlation spectroscopy, Super resolution microscopy, Stimulated emission depletion microscopy, Diffusion, Cell mechanics, Molecules, Calibration, Confocal microscopy, Time resolved spectroscopy, Microscopes

Proceedings Article | 9 March 2020 Presentation
Proc. SPIE. 11244, Multiphoton Microscopy in the Biomedical Sciences XX
KEYWORDS: Molecules, Fluorescence resonance energy transfer, Fluorescence lifetime imaging, Molecular interactions, Luminescence, Electronics, Data analysis, Time resolved spectroscopy, Lung, Tissues

Showing 5 of 37 publications
Proceedings Volume Editor (10)

Showing 5 of 10 publications
Conference Committee Involvement (11)
Single Molecule Spectroscopy and Superresolution Imaging XV
22 January 2022 | San Francisco, California, United States
Single Molecule Spectroscopy and Superresolution Imaging XIV
6 March 2021 | Online Only, California, United States
Single Molecule Spectroscopy and Superresolution Imaging XIII
1 February 2020 | San Francisco, California, United States
Single Molecule Spectroscopy and Superresolution Imaging XII
2 February 2019 | San Francisco, California, United States
Single Molecule Spectroscopy and Superresolution Imaging XI
27 January 2018 | San Francisco, California, United States
Showing 5 of 11 Conference Committees
Course Instructor
SC859: Single Molecule Spectroscopy and Imaging
This course introduces the basics of single molecule fluorescence spectroscopy and imaging under am-bient conditions especially for biologically relevant applications. The first part covers the technological requirements for single molecule fluorescence detection and spectroscopy, covering excitation sources, microscopy optics, optical and mechanical components, single photon detectors, fast photon-counting electronics, data acquisition, processing, visualization and evaluation. The second part focuses on several single-molecule spectroscopic and imaging techniques: fluores-cence-correlation spectroscopy (FCS) and its different variants such as 2-focus FCS, fluorescence life-time correlations spectroscopy (FLCS), fluorescence cross-correlation spectroscopy (FCCS), or photo-electron transfer mediated FCS (PET-FCS); fluorescence intensity distribution analysis (FIDA) alias photon-counting histogram analysis (PCH); single-molecule Förster resonance energy transfer (smFRET); focused and defocused single-molecule imaging and fluorescence lifetime imaging (FLIM). Selected applications will be presented: monitoring conformational changes in proteins, fast conforma-tional fluctuations in peptides, single-molecule motion and reorientation of polymer-embedded chro-mophores and of motor proteins, single molecule and single quantum dot photophysics, and super-resolution fluorescence microscopy.
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