Dr. Franck Bonnier Profile

Dr. Franck Bonnier

Post Doctoral Researcher at Univ Francois Rabelais

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Publications (5)

Proceedings Article | 4 March 2019 Presentation

Identification of diabetic patients via urine analysis by FTIR: preliminary study (Conference Presentation)

Ana Clara Carnevalli, Leonardo Leal, Alexandre Scherma, Camilo Morais, Francis Martin, Franck Bonnier, Matthew Baker, Hugh Byrne, Luís Felipe Chagas e Silva Carvalho, Marcelo Saito Nogueira

Proceedings Volume 10863, 108630E (2019) https://doi.org/10.1117/12.2510175

KEYWORDS: FT-IR spectroscopy, Glucose, Blood, Infrared spectroscopy, Infrared radiation, Attenuated total reflectance, Spectroscopy, Clinical research, Infrared materials, Data modeling

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SPIE Journal Paper | 30 October 2017 Open Access

Raman spectral signatures of cervical exfoliated cells from liquid-based cytology samples

Padraig Kearney, Damien Traynor, Franck Bonnier, Fiona Lyng, John O'Leary, Cara Martin

JBO, Vol. 22, Issue 10, 105008, (October 2017) https://doi.org/10.1117/12.10.1117/1.JBO.22.10.105008

KEYWORDS: Raman spectroscopy, Cell biology, Principal component analysis, Cervical cancer, Proteins, Infrared signatures, Statistical analysis, Tissues, Infrared spectroscopy, Glasses

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Proceedings Article | 19 June 2015 Paper

Raman spectroscopic analysis of oral squamous cell carcinoma and oral dysplasia in the high-wavenumber region

Luis Felipe C. Carvalho, Franck Bonnier, Kate O'Callaghan, Jeff O'Sullivan, Stephen Flint, Lazaro P. Neto, Cláudio A. Soto, Laurita dos Santos, Airton Martin, Hugh Byrne, Fiona Lyng

Proceedings Volume 9531, 953125 (2015) https://doi.org/10.1117/12.2180996

KEYWORDS: Raman spectroscopy, Proteins, Tissues, Cancer, Tissue optics, Spectroscopy, Biopsy, Biological research, Statistical analysis, Biomedical optics

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SPIE Journal Paper | 2 November 2012 Open Access

Raman spectroscopic analysis of human skin tissue sections ex-vivo: evaluation of the effects of tissue processing and dewaxing

Syed Ali, Franck Bonnier, Ali Tfayli, Helen Lambkin, Kathleen Flynn, Vincent McDonagh, Claragh Healy, T. Clive Lee, Fiona Lyng, Hugh Byrne

JBO, Vol. 18, Issue 06, 061202, (November 2012) https://doi.org/10.1117/12.10.1117/1.JBO.18.6.061202

KEYWORDS: Raman spectroscopy, Skin, Tissues, Tissue optics, Biological research, Spectroscopy, Proteins, Collagen, Statistical analysis, Biomedical optics

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Proceedings Article | 9 June 2011 Paper

Collagen matrices as an improved model for in vitro study of live cells using Raman microspectroscopy

F. Bonnier, P. Knief, A. Meade, J. Dorney, K. Bhattacharya, F. Lyng, H. Byrne

Proceedings Volume 8087, 80870F (2011) https://doi.org/10.1117/12.889872

KEYWORDS: Raman spectroscopy, Collagen, In vitro testing, Confocal microscopy, Spectroscopy, Matrices, Imaging spectroscopy, 3D modeling, Molecular spectroscopy, Data modeling

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Due to its high lateral resolution, Raman microspectrsocopy is rapidly becoming an accepted technique for the subcellular imaging of single cells. Although the potential of the technique has frequently been demonstrated, many improvements have still to be realised to enhance the relevancy of the data collected. Although often employed, chemical fixation of cells can cause modifications to the molecular composition and therefore influence the observations made. However, the weak contribution of water to Raman spectra offers the potential to study live cells cultured in vitro using an immersion lens, giving the possibility to record highly specific spectra from cells in their original state. Unfortunately, in common 2-D culture models, the contribution of the substrates to the spectra recorded requires significant data pre-processing causing difficulties in developing automated methods for the correction of the spectra. Moreover, the 2-D in vitro cell model is not ideal and dissimilarities between different optical substrates within in vitro cell cultures results in morphological and functional changes to the cells. The interaction between the cells and their microenvironment is crucial to their behavior but also their response to different external agents such as radiation or anticancer drugs. In order to create an experimental model closer to the real conditions encountered by the cell in vivo, 3-D collagen gels have been evaluated as a substrate for the spectroscopic study of live cells. It is demonstrated that neither the medium used for cell culture nor the collagen gels themselves contribute to the spectra collected. Thus the background contributions are reduced to that of the water. Spectral measurements can be made in full cell culture medium, allowing prolonged measurement times. Optimizations made in the use of collagen gels for live cells analysis by Raman spectroscopy are encouraging and studying live cells within a collagenous microenvironment seems perfectly accessible.

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