Prof. Giulia Rusciano Profile

Prof. Giulia Rusciano

Prof at Univ degli Studi di Napoli Federico II

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Publications (5)

Proceedings Article | 18 April 2021 Presentation + Paper

Nanoporous silver films produced by solid-state dewetting for SERS applications

Giulia Rusciano, Angela Capaccio, Anna Martinez, Antonio Sasso

Proceedings Volume 11772, 117720E (2021) https://doi.org/10.1117/12.2592391

KEYWORDS: Solid state electronics, Silver, Dewetting, Raman spectroscopy, Plasmonics, Plasma, Sputter deposition, Raman scattering, Oxygen, Oxidation

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Proceedings Article | 26 June 2017 Paper

Micro-Raman analysis of glisterings in intraocular lenses

G. Rusciano, A. Martinez, G. Pesce, G. Zito, A. Sasso

Proceedings Volume 10333, 103331A (2017) https://doi.org/10.1117/12.2271830

KEYWORDS: Raman spectroscopy, Polymers, Chemical analysis, Intraocular lenses, Visualization, Micro raman spectroscopy, Optical microscopy, Spectroscopy

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Proceedings Article | 17 July 2015 Paper

Tip enhanced Raman scattering of bacillus subtilis spores

G. Rusciano, G. Zito, G. Pesce, A. Sasso, R. Isticato, E. Ricca

Proceedings Volume 9540, 95400S (2015) https://doi.org/10.1117/12.2183428

KEYWORDS: Raman spectroscopy, Thin film coatings, Raman scattering, Proteins, Molecules, Chemical analysis, Surface enhanced Raman spectroscopy, Atomic force microscopy, Displays, Molecular interactions

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Proceedings Article | 3 May 2013 Paper

Raman-spectroscopy-based biosensing for applications in ophthalmology

Giulia Rusciano, Paola Capriglione, Giuseppe Pesce, Gianluigi Zito, Antonio Del Prete, Giovanni Cennamo, Antonio Sasso

Proceedings Volume 8774, 87740A (2013) https://doi.org/10.1117/12.2018004

KEYWORDS: Raman spectroscopy, Principal component analysis, Tissues, Remote sensing, Ophthalmology, Statistical analysis, Biological research, Proteins, Cell biology, Micro raman spectroscopy

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Cell-based biosensors rely on the detection and identification of single cells as well as monitoring of changes induced by interaction with drugs and/or toxic agents. Raman spectroscopy is a powerful tool to reach this goal, being non-destructive analytical technique, allowing also measurements of samples in aqueous environment. In addition, micro-Raman measurements do not require preliminary sample preparation (as in fluorescence spectroscopy), show a finger-print spectral response, allow a spatial resolution below typical cell sizes, and are relatively fast (few s or even less). All these properties make micro-Raman technique particularly promising for high-throughput on-line analysis integrated in lab-on-a-chip devices. Herein, we demonstrate some applications of Raman analysis in ophthalmology. In particular, we demonstrate that Raman analysis can provide useful information for the therapeutic treatment of keratitis caused by Acanthamoeba Castellanii (A.), an opportunistic protozoan that is widely distributed in the environment and is known to produce blinding keratitis and fatal encephalitis. In particular, by combining Raman analysis with Principal Component Analysis (PCA), we have demonstrated that is possible to distinguish between live and dead cells, enabling, therefore to establish the effectiveness of therapeutic strategies to vanquish the protozoa. As final step, we have analyzed the presence of biochemical differences in the conjunctival epithelial tissues of patients affected by keratitis with respect to healthy people. As a matter of facts, it is possible to speculate some biochemical alterations of the epithelial tissues, rendering more favorable the binding of the protozoan. The epithelial cells were obtained by impression cytology from eyes of both healthy and keratitis-affected individuals. All the samples were analyzed by Raman spectroscopy within a few hours from cells removal from eyes. The results of this analysis are discussed.

Proceedings Article | 3 May 2013 Paper

Surface-enhanced Raman imaging of red blood cell membrane with highly uniform active substrates obtained using block copolymers self-assembly

Gianluigi Zito, Anna Malafronte, Alden Dochshanov, Giulia Rusciano, Finizia Auriemma, Giuseppe Pesce, Claudio De Rosa, Antonio Sasso

Proceedings Volume 8774, 87740B (2013) https://doi.org/10.1117/12.2018213

KEYWORDS: Surface enhanced Raman spectroscopy, Raman spectroscopy, Blood, Glasses, Silver, Silicon, Plasmonics, Nanoparticles, Polymers, Imaging spectroscopy

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