Fluorescence guided surgery has high potential for improved patient outcomes but often lacks quantification of fluorophore depth which is needed to determine surgical margins of solid tumors. To address this need, a dual wavelength excitation approach was applied that capitalizes on the wavelength-dependent attenuation of light in tissue to provide depth information independent of fluorophore concentration. A portable near infrared dual wavelength excitation fluorescence imaging system was built and tested using tissue mimicking phantoms and is currently being tested to determine breast tumor margin status in a first-in-human clinical trial investigating LS301, a novel near infrared tumor-targeted contrast agent.
KEYWORDS: Cancer, Tissues, Thermography, Tumor growth modeling, Thermal modeling, Tumors, 3D modeling, Thermal energy technology, Systems modeling, Real time imaging
Dynamic thermal imaging has improved bulk tissue characterization, but fails to capture subtle thermal property differences in heterogeneous systems. We present focal dynamic thermal imaging (FDTI), a simple, label-free, and high-resolution technology for delineating tissue heterogeneity. Stimulation of focal regions of absorptive materials with a narrow beam, low power, and low cost 405 nm laser locally perturbs the thermal equilibrium. Measurement of phantoms, ex vivo tissue, and in vivo animal models of cancer reveals finite structures of materials and delineates diseased from healthy tissue. Portable FDTI holds promise to capture the heterogeneous nature of malignant tissue.
LS301 is a promising NIR fluorescence probe for targeting brain glioma cancer. The co-localization of LS31 and 5-ALA induced PpIX in vitro and in vivo enables LS301 to guide PpIX fluorescence image surgery. Moreover, LS301 showed no negative impact on 5-ALA-PpIX PDT outcome in brain glioma cells and mouse tumor model. Therefore, the implementation of LS301 and PpIX has potential to improve tumor resection surgery and PDT treatment for better tumor outcomes.
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