Mr. Haixin Huang Profile

Haixin Huang

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Proceedings Article | 17 August 2023 Paper

Doubling the temporal resolution of SOFI with two-color sequence coupling

Haoyuan Qiu, Haixin Huang, Hanzhe Wu, Yihong Ji, Heng Li, Bin Yu, Danni Chen, Junle Qu

Proceedings Volume 12757, 127572P (2023) https://doi.org/10.1117/12.2690221

KEYWORDS: Molecules, Super resolution, Image quality, Fluorophores, Temporal resolution, Fluorescence

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Super-resolution optical fluctuation imaging (SOFI) uses the advantages of computational post-processing to solve the super-resolution problem under high labeling density. Higher-order SOFI requires sufficient fluorescence fluctuation information to reduce artifacts in reconstructed images. However, the length of image sequence is limited for a certain kind of fluorophores, such as the inherent lifetime. In this research, we proposed a method to enhance image quality using double-color acquisitions and then coupling them into sequences. The theoretical basis and the working principle of this method will be explained. Simulations and limitations analysis between typical acquisition and this method will also be given. Simulations demonstrated that this method can improve the overall image quality by about 31.58 % in the 2^nd order SOFI and 56.26 % in the 4^th order SOFI.

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