Ms. Heike Kagel Profile

Heike Kagel

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 19 June 2015 Paper

Plasmonic enhancement in the photoinactivation of Escherichia Coli using rose bengal and gold nanoparticles

Heike Kagel, Julia Honselmann Genannt Humme, Edvaldo Antonio Ribeiro Rosa, Rozane de Fátima Turchiello, Arandi Ginane Bezerra Junior

Proceedings Volume 9531, 953138 (2015) https://doi.org/10.1117/12.2180947

KEYWORDS: Nanoparticles, Plasmonics, Laser ablation, Bacteria, Light emitting diodes, Gold, Absorption, Transmission electron microscopy, Oxygen, Industrial chemicals

Read Abstract +

In the present study we report on the ability of gold nanoparticles (AuNP) to enhance the antimicrobial activity of the photosensitizer Rose Bengal (RB), a very effective singlet oxygen generator. Our experiments were conducted using a suspension of Escherichia Coli in the presence of either RB or a combination of RB and AuNP. Nanoparticles were synthesized by laser ablation in water, which allows high purity, biologically friendly AuNP production, as compared to traditional chemical methods. Several relative concentrations of bacteria, photosensitizes and AuNP were studied. Bacterial survival rates were determined before and after LED light illumination. The phototoxicity of RB with and without AuNP was checked following illumination for 10 and 20 minutes. As a control, the dark toxicity of RB was verified. The results show that the survival rate of bacteria decreases significantly with the increase of RB concentration and illumination time, which is in accordance with previous works. Interestingly, our results also indicate a significant increase in the lethal photosensitization of RB in the presence of AuNP. We propose this effect is due to plasmonic light enhancement, considering the superposition of RB and AuNP absorption spectra, which favors electric field enhancement effects in the presence of AuNP. Similar experiments using the photosensitizer Methylene Blue (MB) allowed us to test our hypothesis for MB did not show any difference in its phototoxicity in the presence of AuNP. We propose this observed synergistic effect could be an effective way for improving photodynamic inactivation of microorganisms.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years