Prof. Herbert Michael Heise Profile

Prof. Herbert Michael Heise

Senior Research Scientist/ Professor at Fachhochschule Südwestfalen

SPIE Involvement:

Conference Program Committee | Author

Area of Expertise:

biospectroscopy , analytical spectroscopy , infrared, near-infrared, Raman, UV/Vis , chemometrics

Websites:

Personal Website

Publications (23)

SPIE Journal Paper | 8 March 2021 Open Access

Systematic stability testing of insulins as representative biopharmaceuticals using ATR FTIR-spectroscopy with focus on quality assurance

Sven Delbeck, Herbert Michael Heise

JBO, Vol. 26, Issue 04, 043007, (March 2021) https://doi.org/10.1117/12.10.1117/1.JBO.26.4.043007

KEYWORDS: Proteins, Deconvolution, Infrared spectroscopy, Statistical analysis, Spectroscopy, Principal component analysis, Absorbance, Biological research, Testing and analysis, Data analysis

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Significance: Bioactive proteins represent the most important component class in biopharmaceutical products for therapeutic applications. Their production is most often biotechnologically realized by genetically engineered microorganisms. For the quality assurance of insulins as representatives of life-saving pharmaceuticals, analytical methods are required that allow more than total protein quantification in vials or batches. Chemical and physical factors such as unstable temperatures or shear rate exposure under storage can lead to misfolding, nucleation, and subsequent fibril forming of the insulins. The assumption is valid that these processes go parallel with a decrease in bioactivity. Aim: Infrared (IR) spectroscopy has been successfully utilized for secondary structure analysis in cases of protein misfolding and fibril formation. Approach: A reliable method for the quantification of the secondary structure changes has been developed using insulin dry-film Fourier-transform IR spectroscopy in combination with the attenuated total reflection (ATR) technique and subsequent data analyses such as band-shift determination, spectral band deconvolution, and principal component analysis. Results: A systematic study of insulin spectra was carried out on model insulin specimens, available either as original formulations or as hormones purified by ultrafiltration. Insulin specimens were stored at different temperatures, i.e., 0°C, 20°C, and 37°C, respectively, for up to three months. Weekly ATR-measurements allowed the monitoring of hormone secondary structure changes, which are supposed to be negatively correlated with insulin bioactivity. Conclusions: It could be shown that IR-ATR spectroscopy offers a fast and reliable analytical method for the determination of secondary structural changes within insulin molecules, as available in pharmaceutical insulin formulations and therefore challenges internationally established measurement techniques for quality control regarding time, costs, and effort of analysis.

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Proceedings Article | 21 February 2020 Paper

Molecular monitoring of different insulins using FTIR-ATR spectroscopy for pharmaceutical quality control

S. Delbeck, H. M. Heise

Proceedings Volume 11236, 112360V (2020) https://doi.org/10.1117/12.2546170

KEYWORDS: Proteins, Spectroscopy, Deconvolution, Infrared spectroscopy, Biological research, Infrared radiation, Absorbance, Principal component analysis, Molecular spectroscopy, Attenuated total reflectance

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Proceedings Article | 21 February 2020 Paper

Development of an insulin activity test based on cell culture monitoring by microdialysis and infrared spectrometry

A. Krolinski, S. Delbeck, Y. Dederich, S. Stoppelkamp, H. M. Heise

Proceedings Volume 11236, 112360O (2020) https://doi.org/10.1117/12.2546154

KEYWORDS: Glucose, Infrared spectroscopy, Proteins, Biological research, Testing and analysis

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Proceedings Article | 27 February 2019 Paper

Applications of tapered flat silver halide fibers chemically modified by 12-mercaptododecanoic acid NHS ester for infrared biospectroscopy with prospects for medical diagnostics

S. Delbeck, B. Schmitz, A. Nabers, K. Gerwert, A. Habermehl, U. Lemmer, H. M. Heise

Proceedings Volume 10872, 108720T (2019) https://doi.org/10.1117/12.2511023

KEYWORDS: Optical fibers, Printing, Proteins, Infrared radiation, Nanoparticles, Sensors, Absorption

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Proceedings Article | 20 February 2018 Paper

Evaluation and benchmarking of an EC-QCL-based mid-infrared spectrometer for monitoring metabolic blood parameters in critical care units

M. Grafen, S. Delbeck, H. Busch, H. Heise, A. Ostendorf

Proceedings Volume 10501, 105010A (2018) https://doi.org/10.1117/12.2289625

KEYWORDS: Spectroscopy, Glucose, Quantum cascade lasers, Infrared spectroscopy, FT-IR spectroscopy, Blood, Calibration, Mid-IR, Infrared radiation

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Proceedings Article | 13 February 2018 Presentation + Paper

Applications of tapered flat silver halide fiber elements for infrared biospectroscopy with aspects of optical stability and biocompatibility

Sven Delbeck, Lukas Küpper, Herbert Heise

Proceedings Volume 10488, 104880Q (2018) https://doi.org/10.1117/12.2290967

KEYWORDS: Silver, Glucose, Sensors, Chemical elements, Fiber optics sensors, Spectroscopy, Blood, Absorbance, Infrared radiation, Infrared spectroscopy

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Proceedings Article | 17 February 2017 Presentation + Paper

Monitoring of interstitial buffer systems using micro-dialysis and infrared spectrometry

H. Heise, L. Cocchieri, T. Vahlsing, D. Ihrig, J. Elm

Proceedings Volume 10072, 100720E (2017) https://doi.org/10.1117/12.2253139

KEYWORDS: Carbon dioxide, Infrared spectroscopy, Blood, Sensing systems, Infrared radiation, Spectroscopy, Point-of-care devices, Carbon monoxide, Absorbance, Absorption, Proteins, Gases

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Proceedings Article | 17 February 2017 Presentation + Paper

Reliable glucose monitoring by ex-vivo blood microdialysis and infrared spectrometry for patients in critical care

Thorsten Vahlsing, Sven Delbeck, Janpeter Budde, Dieter Ihrig, Steffen Leonhardt, H. Michael Heise

Proceedings Volume 10072, 100720D (2017) https://doi.org/10.1117/12.2253086

KEYWORDS: Infrared spectroscopy, Glucose, Blood, Infrared radiation, Spectroscopy, Spectral calibration, Calibration, Sensing systems, Infrared sensors, Biosensors, Absorbance, Statistical analysis, Microfluidics, Sensors

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Proceedings Article | 7 March 2016 Presentation + Paper

Combination of micro-dialysis and infrared spectroscopy: a multianalyte assay for accurate biofluid analysis and patient monitoring

Thorsten Vahlsing, Sven Delbeck, Janpeter Budde, Dieter Ihrig, H. Michael Heise

Proceedings Volume 9704, 97040R (2016) https://doi.org/10.1117/12.2214636

KEYWORDS: Infrared spectroscopy, Glucose, Biological research, Spectroscopy, Calibration, Blood, Quantitative analysis, Clinical research, Absorbance, Microfluidics, Sensors, Sodium

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Proceedings Article | 4 March 2016 Presentation + Paper

Characterization of a multi-module tunable EC-QCL system for mid-infrared biofluid spectroscopy for hospital use and personalized diabetes technology

M. Grafen, K. Nalpantidis, A. Ostendorf, D. Ihrig, H. Heise

Proceedings Volume 9715, 97150T (2016) https://doi.org/10.1117/12.2213635

KEYWORDS: Glucose, Calibration, Sensing systems, Infrared spectroscopy, Quantum cascade lasers, Infrared technology, Infrared radiation, Spectroscopy, Blood, Point-of-care devices, Sensors, Absorbance, Spectral resolution, Pulsed laser operation, Laser systems engineering, Mid-IR

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Proceedings Article | 4 March 2016 Paper

Performance testing of a mid-infrared spectroscopic system for clinical chemistry applications utilising an ultra-broadband tunable EC-QCL radiation source

M. Grafen, K. Nalpantidis, D. Ihrig, H. Heise, A. Ostendorf

Proceedings Volume 9715, 971517 (2016) https://doi.org/10.1117/12.2213538

KEYWORDS: Glucose, Quantum cascade lasers, Absorbance, Factor analysis, Mid-IR, Spectroscopy, Mirrors, Sensors, Pulsed laser operation, Laser systems engineering

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Proceedings Article | 15 July 2015 Paper

Mid-infrared spectroscopic characterisation of an ultra-broadband tunable EC-QCL system intended for biomedical applications

T. Vahlsing, H. Moser, M. Grafen, K. Nalpantidis, M. Brandstetter, H. Heise, B. Lendl, S. Leonhardt, D. Ihrig, A. Ostendorf

Proceedings Volume 9537, 953713 (2015) https://doi.org/10.1117/12.2183952

KEYWORDS: Spectral resolution, Quantum cascade lasers, Pulsed laser operation, Spectroscopy, Blood, Glucose, Mid-IR, FT-IR spectroscopy, Plasma, Calibration

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Proceedings Article | 9 March 2015 Paper

Ex-vivo glucose sensors using micro-dialysis: importance of on-line recovery rate determination by multi-analyte infrared spectrometry

Thorsten Vahlsing, Sven Delbeck, Janpeter Budde, Lars Cocchieri, Dieter Ihrig, Steffen Leonhardt, H. Heise

Proceedings Volume 9332, 933209 (2015) https://doi.org/10.1117/12.2080289

KEYWORDS: Glucose, Infrared spectroscopy, Blood, Microfluidics, Spectroscopy, Optical filters, Absorbance, Spectral resolution, Infrared radiation, Sensors

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Proceedings Article | 28 February 2014 Paper

Influence of spectral bandwidth limitations of tuneable external-cavity based quantum cascade laser systems for clinical biofluid analysis

H. Michael Heise, Thorsten Vahlsing

Proceedings Volume 8951, 895108 (2014) https://doi.org/10.1117/12.2040173

KEYWORDS: Glucose, Blood, Plasma, Calibration, Absorbance, Quantum cascade lasers, Urea, Infrared spectroscopy, Proteins, Point-of-care devices

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Proceedings Article | 22 February 2008 Paper

Microdialysis based monitoring of subcutaneous interstitial and venous blood glucose in Type 1 diabetic subjects by mid-infrared spectrometry for intensive insulin therapy

H. Michael Heise, Venkata Radhakrishna Kondepati, Uwe Damm, Michael Licht, Franz Feichtner, Julia Katharina Mader, Martin Ellmerer

Proceedings Volume 6863, 686308 (2008) https://doi.org/10.1117/12.772050

KEYWORDS: Glucose, Blood, Sensors, Spectroscopy, Microfluidics, Calibration, Mid-IR, Infrared spectroscopy, Infrared sensors, Absorbance

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SPIE Journal Paper | 1 March 2007 Open Access

Bedside monitoring of subcutaneous interstitial glucose in healthy individuals using microdialysis and infrared spectrometry

Herbert Heise, Uwe Damm, Manfred Bodenlenz, Venkata Kondepati, Gerd Kohler, Martin Ellmerer

JBO, Vol. 12, Issue 02, 024004, (March 2007) https://doi.org/10.1117/12.10.1117/1.2714907

KEYWORDS: Glucose, Calibration, Blood, Spectroscopy, Infrared spectroscopy, Data modeling, Microfluidics, Sensors, Absorbance, Plasma

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Proceedings Article | 14 February 2007 Paper

Bedside monitoring of subcutaneous interstitial glucose in type 1 diabetic subjects using microdialysis and infrared spectrometry with optimal correlation to blood glucose concentrations

H. Michael Heise, Uwe Damm, Venkata Kondepati, J. Mader, M. Ellmerer

Proceedings Volume 6445, 644502 (2007) https://doi.org/10.1117/12.702492

KEYWORDS: Glucose, Blood, Infrared spectroscopy, Calibration, Absorbance, Spectroscopy, Infrared radiation, Plasma, Statistical analysis, Microfluidics

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Proceedings Article | 27 February 2006 Paper

Recent progress in noninvasive diabetes screening by diffuse reflectance near-infrared skin spectroscopy

H. Heise, S. Haiber, M. Licht, D. Ihrig, C. Moll, M. Stuecker

Proceedings Volume 6093, 609310 (2006) https://doi.org/10.1117/12.650825

KEYWORDS: Skin, Fiber optics, Proteins, Tissue optics, Blood, Spectroscopy, Glucose, Diffuse reflectance spectroscopy, Near infrared spectroscopy, Fiber optics tests

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Proceedings Article | 27 February 2006 Paper

Reliable long-term continuous blood glucose monitoring for patients in critical care using microdialysis and infrared spectrometry

H. Michael Heise, Uwe Damm, Venkata Kondepati

Proceedings Volume 6093, 609303 (2006) https://doi.org/10.1117/12.650833

KEYWORDS: Glucose, Blood, Calibration, Infrared spectroscopy, Spectroscopy, Absorbance, Infrared radiation, Sensors, Data modeling, In vivo imaging

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Proceedings Article | 24 April 1998 Paper

Blood glucose assays based on infrared spectroscopy: alternatives for medical diagnostics

Herbert Heise, Andreas Bittner

Proceedings Volume 3257, (1998) https://doi.org/10.1117/12.306073

KEYWORDS: Glucose, Blood, Absorbance, Calibration, Spectroscopy, Plasma, Spectral calibration, Infrared spectroscopy, Tissue optics, Mid-IR

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Proceedings Article | 31 January 1994 Paper

Effect of data pretreatment on the noninvasive blood glucose measurement by diffuse reflectance NIR spectroscopy

Herbert Heise, Ralf Marbach

Proceedings Volume 2089, (1994) https://doi.org/10.1117/12.166662

KEYWORDS: Glucose, Diffuse reflectance spectroscopy, Blood, Spectroscopy, Reflectance spectroscopy, Calibration, Near infrared, Near infrared spectroscopy, Tolerancing, Reflectivity

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Proceedings Article | 1 March 1992 Paper

Multivariate determination of blood substrates in human plasma by FT-NIR spectroscopy

Herbert Heise, Ralf Marbach, Th. Koschinsky, F. Gries

Proceedings Volume 1575, (1992) https://doi.org/10.1117/12.56396

KEYWORDS: Plasma, Calibration, Proteins, Glucose, Urea, Blood, Plasma spectroscopy, FT-NIR spectroscopy, Statistical analysis, Spectroscopy

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Proceedings Article | 1 March 1992 Paper

Novel design of an ellipsoidal diffuse-reflectance accessory with improved throughput

Ralf Marbach, Herbert Heise

Proceedings Volume 1575, (1992) https://doi.org/10.1117/12.56397

KEYWORDS: Mirrors, Diffuse reflectance spectroscopy, Infrared spectroscopy, Tissue optics, Tissues, Statistical analysis, Sensors, Solids, Infrared radiation, Testing and analysis

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