Mr. Hsuan Ou-Yang Profile

Hsuan Ou-Yang

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 4 March 2019 Presentation

In vivo three dimensional cuticle intact Drosophila brain imaging using laser scanning optical resolution photoacoustic microscopy

Kai-Yao Chang, Hsuan Ou-Yang, Pei-Shan Ho, Shun-Chi Wu, Yen-Yin Lin, Ann-Shyn Chiang, Meng-Lin Li

Proceedings Volume 10878, 108781Y (2019) https://doi.org/10.1117/12.2508091

KEYWORDS: Brain, In vivo imaging, 3D image processing, 3D photoacoustic microscopy, Brain imaging, Laser scanners, Optical resolution, Photoacoustic microscopy, Confocal microscopy, Surgery

Read Abstract +

To study the structure and functions of the Drosophila brain, confocal microscopy is commonly used. However, surgical removal of the head cuticle of Drosophila is required because the cuticle hinders both the optical excitation and detection. Such invasive surgery may affect brain functions and prohibits long term monitoring. Targeting to the unmet need of surgery free procedure, here we propose laser scanning optical resolution photoacoustic microscopy (LSOR-PAM) for in vivo three dimensional cuticle intact Drosophila brain imaging. Cuticle intact Drosophila brains with cells in optic lobes expressing fluorescent protein DsRed, which serves as an optical absorber and thus a photoacoustic signal source, were imaged. Acquired in vivo 3D LSOR-PAM cuticle-intact brain images were cross-validated using their confocal microscopic counterparts with the cuticles being surgically removed. Acoustic and optical attenuation of the cuticles and degradation in spatial resolution caused by the cuticles were also measured, which explains the reason why LSOR-PAM outperforms confocal microscopy for cuticle intact brains. In addition, the optical absorption bleaching of DsRed expressing optic lobes as a function of the number of the repeated experiments was measured, verifying the LSOR-PAM long-term monitoring capability. In summary, we have demonstrated 3D LSOR-PAM of the Drosophila brain without invasive surgery for the first time. The focus of the future work will be on ways to explore its functional imaging capability on the cuticle intact Drosophila brain.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years