Dr. Hudson Zanin Profile

Dr. Hudson Zanin

at Univ of Bristol

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Proceedings Article | 19 June 2015 Paper

Micro energy-dispersive x-ray fluorescence spectrometry study of dentin coating with nanobiomaterials

Luís Eduardo Silva Soares, Sídnei Nahorny, Fernanda Roberta Marciano, Hudson Zanin, Anderson de Oliveira Lobo

Proceedings Volume 9531, 95313B (2015) https://doi.org/10.1117/12.2180965

KEYWORDS: Calcium, Composites, X-ray fluorescence spectroscopy, Spectroscopy, Coating, Fluorine, Fluorescence spectroscopy, Teeth, Oxides, Minerals

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New biomaterials such as multi-walled carbon nanotubes oxide/graphene oxide (MWCNTO/GO), nanohydroxyapatite (nHAp) and combination of them together or not to acidulated phosphate fluoride gel (F) have been tested as protective coating before root dentin erosion. Fourteen bovine teeth were cleaned, polished, divided into two parts (n=28) and assigned to seven groups: (Control) – without previous surface treatment; F treatment; nHAp; MWCNTO/GO; F+nHAp; F+MWCNTO/GO and F+MWCNTO/GO/nHAp composites. Each sample had two sites of pre-treatments: acid etched area and an area without treatment. After the biomaterials application, the samples were submitted to six cycles (demineralization: orange juice, 10 min; remineralization: artificial saliva, 1 h). Micro energy-dispersive X-ray fluorescence spectrometry (μ-EDXRF) mapping area analyses were performed after erosive cycling on both sites (n=84). μ-EDXRF mappings showed that artificial saliva and MWCNTO/GO/nHAp/F composite treatments produced lower dentin demineralization than in the other groups. Exposed dentin tubules allowed better interaction of nanobiomaterials than in smear layer covered dentin. Association of fluoride with other biomaterials had a positive influence on acid etched dentin. MWCNTO/GO/nHAp/F composite treatment resulted in levels of demineralization similar to the control group.

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