Prof. Ilker Hacihaliloglu Profile

Prof. Ilker Hacihaliloglu

at Univ of British Columbia

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Publications (9)

Proceedings Article | 10 April 2023 Presentation + Paper

Augmenting endometriosis analysis from ultrasound data using deep learning

Adrian Balica, Jennifer Dai, Kayla Piiwaa, Xiao Qi, Ashlee Green, Nancy Phillips, Susan Egan, Ilker Hacihaliloglu

Proceedings Volume 12470, 124700O (2023) https://doi.org/10.1117/12.2653940

KEYWORDS: Ultrasonography, Deep learning, Diagnostics, Education and training, Cross validation, Laparoscopy, Image processing, Image classification, Network architectures, Machine learning

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SPIE Journal Paper | 30 May 2022 Open Access

Förster resonance energy transfer efficiency of the vinculin tension sensor in cultured primary cortical neuronal growth cones

Marina Ayad, Timothy Mahon, Mihir Patel, Marina Cararo-Lopes, Ilker Hacihaliloglu, Bonnie Firestein, Nada Boustany

NPh, Vol. 9, Issue 02, 025002, (May 2022) https://doi.org/10.1117/12.10.1117/1.NPh.9.2.025002

KEYWORDS: Fluorescence resonance energy transfer, Neurons, Image segmentation, Sensors, Resonance energy transfer, Energy efficiency, Neurophotonics, Image registration, Glasses, Optical filters

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Significance: Interaction of neurons with their extracellular environment and the mechanical forces at focal adhesions and synaptic junctions play important roles in neuronal development. Aim: To advance studies of mechanotransduction, we demonstrate the use of the vinculin tension sensor (VinTS) in primary cultures of cortical neurons. VinTS consists of TS module (TSMod), a Förster resonance energy transfer (FRET)-based tension sensor, inserted between vinculin’s head and tail. FRET efficiency decreases with increased tension across vinculin. Approach: Primary cortical neurons cultured on glass coverslips coated with poly-d-lysine and laminin were transfected with plasmids encoding untargeted TSMod, VinTS, or tail-less vinculinTS (VinTL) lacking the actin-binding domain. The neurons were imaged between day in vitro (DIV) 5 to 8. We detail the image processing steps for calculation of FRET efficiency and use this system to investigate the expression and FRET efficiency of VinTS in growth cones. Results: The distribution of fluorescent constructs was similar within growth cones at DIV 5 to 8. The mean FRET efficiency of TSMod (28.5 ± 3.6 % ) in growth cones was higher than the mean FRET efficiency of VinTS (24.6 ± 2 % ) and VinTL (25.8 ± 1.8 % ) (p < 10 − 6). While small, the difference between the FRET efficiency of VinTS and VinTL was statistically significant (p < 10 − 3), suggesting that vinculin is under low tension in growth cones. Two-hour treatment with the Rho-associated kinase inhibitor Y-27632 did not affect the mean FRET efficiency. Growth cones exhibited dynamic changes in morphology as observed by time-lapse imaging. VinTS FRET efficiency showed greater variance than TSMod FRET efficiency as a function of time, suggesting a greater dependence of VinTS FRET efficiency on growth cone dynamics compared with TSMod. Conclusions: The results demonstrate the feasibility of using VinTS to probe the function of vinculin in neuronal growth cones and provide a foundation for studies of mechanotransduction in neurons using this tension probe.

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Proceedings Article | 3 March 2017 Paper

3D/2D image registration method for joint motion analysis using low-quality images from mini C-arm machines

Soheil Ghafurian, Ilker Hacihaliloglu, Dimitris Metaxas, Virak Tan, Kang Li

Proceedings Volume 10135, 101350B (2017) https://doi.org/10.1117/12.2254678

KEYWORDS: Image registration, Motion analysis, Fluoroscopy, Kinematics, 3D metrology, Bone, Image analysis, Surgery, Video, Imaging systems, 3D modeling, Image segmentation, Image quality, 3D image processing, Medical imaging

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SPIE Journal Paper | 2 December 2016

Structure-enhanced local phase filtering using L₀ gradient minimization for efficient semiautomated knee magnetic resonance imaging segmentation

Mikhiel Lim, Ilker Hacihaliloglu

JMI, Vol. 3, Issue 04, 044503, (December 2016) https://doi.org/10.1117/12.10.1117/1.JMI.3.4.044503

KEYWORDS: Image segmentation, Bone, Image filtering, Magnetic resonance imaging, Tissues, Cartilage, Surgery, 3D image processing, 3D modeling, Image enhancement

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Proceedings Article | 24 March 2016 Paper

Segmentation of knee MRI using structure enhanced local phase filtering

Mikhiel Lim, Ilker Hacihaliloglu

Proceedings Volume 9785, 97853B (2016) https://doi.org/10.1117/12.2216568

KEYWORDS: Image segmentation, Bone, Magnetic resonance imaging, Tissues, Natural surfaces, Surgery, Interfaces, Control systems, Image processing algorithms and systems, Image enhancement, Image processing, 3D modeling

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Proceedings Article | 18 March 2015 Paper

3D/2D image registration using weighted histogram of gradient directions

Soheil Ghafurian, Ilker Hacihaliloglu, Dimitris Metaxas, Virak Tan, Kang Li

Proceedings Volume 9415, 94151Z (2015) https://doi.org/10.1117/12.2081316

KEYWORDS: 3D modeling, Image registration, Bone, 3D image processing, Distance measurement, 3D image reconstruction, Computed tomography, Medical imaging, Image restoration, Video

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Proceedings Article | 18 March 2015 Paper

Scaphoid fracture fixation: localization of bones through statistical model to ultrasound registration

Emran Mohammad Abu Anas, Abtin Rasoulian, Paul St. John, David Pichora, Parvin Mousavi, Victoria Lessoway, Alexander Seitel, Ilker Hacihaliloglu, Robert Rohling, Purang Abolmaesumi

Proceedings Volume 9415, 94150P (2015) https://doi.org/10.1117/12.2081578

KEYWORDS: Bone, 3D modeling, Image registration, Statistical analysis, Ultrasonography, Statistical modeling, Clouds, Surgery, Navigation systems, Injuries

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Proceedings Article | 12 March 2014 Paper

A global CT to US registration of the lumbar spine

Simrin Nagpal, Ilker Hacihaliloglu, Tamas Ungi, Abtin Rasoulian, Jill Osborn, Victoria Lessoway, Robert Rohling, Daniel Borschneck, Purang Abolmaesumi, Parvin Mousavi

Proceedings Volume 9036, 90362O (2014) https://doi.org/10.1117/12.2043934

KEYWORDS: Spine, Bone, Image registration, Computed tomography, Ultrasonography, Transducers, Data acquisition, Visualization, Global Positioning System, Sensors

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Proceedings Article | 13 March 2010 Paper

3D ultrasound volume stitching using phase symmetry and harris corner detection for orthopaedic applications

Rupin Dalvi, Ilker Hacihaliloglu, Rafeef Abugharbieh

Proceedings Volume 7623, 762330 (2010) https://doi.org/10.1117/12.844608

KEYWORDS: Image registration, 3D image processing, Bone, Ultrasonography, Image processing, Electronic filtering, Corner detection, Detection and tracking algorithms, 3D scanning, Fetus

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Showing 5 of 9 publications

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