Staining technique and NIR LED based setup has been developed for study of photodynamic action (PDA) of Indocyanine Green (ICG) on Staphylococcus Aureus. ICG appeared sufficiently effective PDA photosensitizer acceptable for grampositive bacterial cultures effective suppression. The efficiency of PDA was estimated as 89%.
We studied photodynamic action of LED radiation (660 nm) sensitized by Methylene Blue on bacteria strain Staphylococcus 209 P. Method efficiency was compared with photodynamic action of He-Ne laser radiation (632,8 nm). Methylene Blue was proved to possess good photosensitizing properties and its application in photodynamic therapy of pyoinflammatory diseases was found advantageous.
The work is devoted to an analysis of pre-clinical and clinical experiments on photodynamic action of HeNe laser radiation in aggregate with a cation thiazinium dye Methylene Blue (MB) on a mix of pathogenic and conditionally pathogenic aerobic bacteria being activators of pyoinflammatory diseases of oral cavity. Concentration of photosensitizes at which there is no own bactericidal influence on dying microflora, and parameters of influence at which the efficiency of irradiated microflora defeat reaches 99 % are determined.
The work is devoted to an analysis of pre-clinical and clinical experiments on photodynamic action of HeNe laser radiation in aggregate with a cation thiazinium dye Methylene Blue (MB) on a mix of pathogenic and conditionally pathogenic aerobic bacteria being activators of pyoinflammatory diseases of oral cavity. Concentration of photosensitizes at which there is no own bactericidal influence on dying microflora, and parameters of influence at which the efficiency of irradiated microflora defeat reaches 99% are determined.
We have studied photodynamic action of He-Ne laser radiation on cultures of Staphylococcus (strain 209 P), Streptococcus anhaemolyticus, and total microflora of dental deposit been sensitized by methylene blue. The concentration of the dye was varied from 0.001% to 0.1%, radiation power density was 100 divided by 2300 mW/cm2. Irradiated strain was put into thermostat for 48 hours and the number of colonies was counted and analyzed.
We have studied photodynamic action of He-Ne laser radiation on cultures of Staphylococcus and Streptococcus anhaemolyticus been sensitized by methylene blue. The concentration of the dye was varied form 0.001 percent to 0.1 percent and radiation power density from 75 to 200 mW/cm2. Irradiated strain was put into thermostat for 24 hours, then the number of colonies was counted and analyzed.
We have studied photodynamic action of He-Ne laser radiation on cultures of Staphylococcus (strain 209 P), Streptococcus anhaemolyticus, and total microflora of dental deposit been sensitized by methylene blue. The concentration of the dye was varied from 0.001% to 0.1%, radiation power density was 100 mW/cm2. Irradiated strain was put into thermostat for 24 hours, then the number of colonies was counted and analyzed.
The device, offered for reviewing, was designed and developed for photodynamic therapy of oral cavity mucous diseases and for laboratory experiments on the red light influence on the bacterial colonies in presence of a dye. The device has rather simple construction, it is cheap but convenience in use.
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