Dr. Irene Costantini Profile

Dr. Irene Costantini

Research Fellow at LENS - Lab Europeo di Spettroscopie Non-Lineari

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Author

Publications (23)

Proceedings Article | 16 May 2023 Presentation + Paper

High-speed light-sheet microscopy imaging and data post-processing using custom hardware and software solutions

Giacomo Mazzamuto, Ludovico Silvestri, Giuseppe Sancataldo, Vladislav Gavryusev, Marina Scardigli, Irene Costantini, Francesco Pavone

Proceedings Volume 12385, 1238504 (2023) https://doi.org/10.1117/12.2650374

KEYWORDS: Brain, Data acquisition, Cameras, Microscopy, Microscopes, Open source software, Image compression, Equipment, Acoustooptic tunable filters, Biological samples

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Proceedings Article | 15 March 2023 Presentation + Paper

High-resolution human brain 3D reconstruction with light-sheet fluorescence microscopy

Irene Costantini, Marina Scardigli, Josephine Ramazzotti, Niamh Brady, Franco Cheli, Giacomo Mazzamuto, Filippo Castelli, Curzio Checcucci, Ludovico Silvestri, Paolo Frasconi, Francesco Saverio Pavone

Proceedings Volume 12365, 1236503 (2023) https://doi.org/10.1117/12.2649323

KEYWORDS: Brain, Neuroimaging, Microscopes, Fluorescence microscopy, Fluorescence, Clouds, Brain mapping, Neuroscience, Neurons

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Proceedings Article | 30 May 2022 Poster

3D molecular phenotyping of the human brain Broca’s area using light-sheet fluorescence microscopy

Marina Scardigli, Irene Costantini, Niamh Brady, Mohamed Baghdad, Josephine Ramazzotti, Giacomo Mazzamuto, Filippo Maria Castelli, Curzio Checcucci, Ludovico Silvestri, Paolo Frasconi, Francesco Saverio Pavone

Proceedings Volume PC12144, PC1214415 (2022) https://doi.org/10.1117/12.2620512

KEYWORDS: Brain, Luminescence, Neurons, Microscopy, Brain mapping, Tissues, Microscopes, Data acquisition, Bandpass filters, Associative arrays

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SPIE Journal Paper | 27 April 2022 Open Access

Neurophotonic tools for microscopic measurements and manipulation: status report

Ahmed Abdelfattah, Srinivasa Rao Allu, Robert Campbell, Xiaojun Cheng, Tomáš Cižmár, Irene Costantini, Valentina Emiliani, Natalie Fomin-Thunemann, Ariel Gilad, Tomás Fernández Alfonso, Christopher G. Ferri, Andrew Harris, Elizabeth M. Hillman, Matthew Holt, Kivilcim Kiliç, Evan Miller, Rickson Mesquita, K.M. Naga Srinivas Nadella, U. Valentin Nägerl, Citlali Perez Campos, Francesca Puppo, Shy Shoham, R. Angus Silver, Vivek Srinivasan, Martin Thunemann, Lei Tian, Sergei Vinogradov, Flavia Vitale, Hana Uhlířová, Chris Xu, Mu-Han Yang, Yongxin Zhao, Sapna Ahuja, Taner Akkin, Joshua Brake, David Boas, Erin Buckley, Anderson Chen, Massimo De Vittorio, Anna Devor, Patrick Doran, Mirna El Khatib, Yeshaiahu Fainman, Xue Han, Ute Hochgeschwender, Na Ji, Evelyn Lake, Lei Li, Tianqi Li, Philipp Mächler, Yusuke Nasu, Axel Nimmerjahn, Petra Ondrácková, Francesco Pavone, Darcy Peterka, Filippo Pisano, Ferruccio Pisanello, Bernardo Sabatini, Sanaz Sadegh, Sava Sakadžic, Sanaya Shroff, Ruth Sims, Spencer Smith, Lin Tian, Thomas Troxler, Antoine Valera, Alipasha Vaziri, Lihong Wang, Changhuei Yang, Gary Yellen, Ofer Yizhar

NPh, Vol. 9, Issue S1, 013001, (April 2022) https://doi.org/10.1117/12.10.1117/1.NPh.9.S1.013001

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Proceedings Article | 5 March 2021 Presentation

Feature enhancement volumetric visualization of human brain sample with serial sectioning spectral domain optical coherence tomography

Jiarui Yang, Shuaibin Chang, Ichun Anderson Chen, Divya Varadarajan, Hui Wang, Caroline Magnain, Bruce Fischl, Irene Costantini, Francesco Pavone, Ann McKee, Shih-Chi Chen, David Boas

Proceedings Volume 11629, 116290Y (2021) https://doi.org/10.1117/12.2577771

KEYWORDS: Optical coherence tomography, Brain, Visualization, Signal attenuation, Tissue optics, Tissues, Structured optical fibers, Speckle, Signal to noise ratio, Scattering

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SPIE Journal Paper | 21 October 2020 Open Access

Improving the characterization of ex vivo human brain optical properties using high numerical aperture optical coherence tomography by spatially constraining the confocal parameters

Jiarui Yang, Ichun Anderson Chen, Shuaibin Chang, Jianbo Tang, Blaire Lee, Kivilcim Kılıç, Smrithi Sunil, Hui Wang, Divya Varadarajan, Caroline Magnain, Shih-Chi Chen, Irene Costantini, Francesco Saverio Pavone, Bruce Fischl, David Boas

NPh, Vol. 7, Issue 04, 045005, (October 2020) https://doi.org/10.1117/12.10.1117/1.NPh.7.4.045005

KEYWORDS: Scattering, Brain, Optical properties, Optical coherence tomography, Tissue optics, Neuroimaging, Confocal microscopy, Tissues, Statistical modeling, Statistical analysis

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Proceedings Article | 1 April 2020 Presentation + Paper

Fast volumetric mapping of human brain slices

Luca Pesce, Annunziatina Laurino, Vladislav Gavryusev, Giacomo Mazzamuto, Giuseppe Sancataldo, Marina Scardigli, Matteo Roffilli, Ludovico Silvestri, Irene Costantini, Francesco Saverio Pavone

Proceedings Volume 11360, 113600A (2020) https://doi.org/10.1117/12.2555644

KEYWORDS: Brain, Neuroimaging, Microscopy, Tissues, Luminescence, Brain mapping, Neurons, Refractive index, Switches, Tissue optics

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Proceedings Article | 21 February 2020 Presentation + Paper

Fast volumetric mapping of human brain slices

Luca Pesce, Annunziatina Laurino, Vladislav Gavryusev, Giacomo Mazzamuto, Giuseppe Sancataldo, Marina Scardigli, Matteo Roffilli, Ludovico Silvestri, Irene Costantini, Francesco Saverio Pavone

Proceedings Volume 11226, 112260B (2020) https://doi.org/10.1117/12.2544133

KEYWORDS: Brain, Brain mapping, Neuroimaging, Tissues, Refractive index, Microscopy, Neurons, Tissue optics, Tissue clearing

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Proceedings Article | 22 July 2019 Paper

Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method

Irene Costantini, Giacomo Mazzamuto, Annunziatina Laurino, Erica Lazzeri, Leonardo Sacconi, Mattia Neri, Andrea Simonetto, Matteo Roffilli, Ludovico Silvestri, Francesco S. Pavone

Proceedings Volume 11076, 110760B (2019) https://doi.org/10.1117/12.2527096

KEYWORDS: Brain, Tissues, Analytical research, Neuroimaging, Tissue optics, Image resolution, Biological research

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Proceedings Article | 14 March 2018 Presentation

Mapping the quantitative cytoarchitecture of the whole mouse brain by light-sheet microscopy and digital brain atlasing (Conference Presentation)

Ludovico Silvestri, Antonino Paolo Di Giovanna, Giacomo Mazzamuto, Francesco Orsini, Irene Costantini, Jan Bjaalie, Paolo Frasconi, Francesco Saverio Pavone

Proceedings Volume 10481, 104810H (2018) https://doi.org/10.1117/12.2286616

KEYWORDS: Brain, Brain mapping, Neuroimaging, Microscopy, Image analysis, Brain imaging, Image quality, Tissue clearing, Image registration, 3D image reconstruction

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Proceedings Article | 14 March 2018 Presentation

Whole heart cytoarchitecture at micron-scale resolution (Conference Presentation)

Erica Lazzeri, Irene Costantini, Cecilia Ferrantini, Samantha Cannazzaro, Giacomo Mazzamuto, Claudia Crocini, Raffaele Coppini, Leonardo Bocchi, Elisabetta Cerbai, Corrado Poggesi, Francesco Pavone, Leonardo Sacconi

Proceedings Volume 10471, 104710L (2018) https://doi.org/10.1117/12.2288553

KEYWORDS: Heart, Collagen, 3D modeling, Tissues, Associative arrays, Genetics, Tissue clearing, Optical microscopy, Second-harmonic generation, Action potentials

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Proceedings Article | 28 July 2017 Paper

Correlative polarized light imaging and two-photon fluorescence microscopy for 3D myelinated fibers reconstruction

Irene Costantini, Miriam Menzel, Ludovico Silvestri, Nicole Schubert, Markus Axer, Katrin Amunts, Francesco Pavone

Proceedings Volume 10413, 1041306 (2017) https://doi.org/10.1117/12.2284328

KEYWORDS: 3D image processing, Confocal microscopy, Two photon imaging, Two photon excitation microscopy, Microscopy

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Proceedings Article | 28 July 2017 Paper

Optimal staining and clearing protocol for whole mouse brain vasculature imaging with light-sheet microscopy

Antonino Paolo Di Giovanna, Alessandro Tibo, Ludovico Silvestri, Marie Caroline Müllenbroich, Irene Costantini, Leonardo Sacconi, Paolo Frasconi, Francesco Saverio Pavone

Proceedings Volume 10414, 104140N (2017) https://doi.org/10.1117/12.2285044

KEYWORDS: Vascular imaging, Neuroimaging, Brain, Microscopy, Tissue clearing

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Proceedings Article | 27 April 2016 Presentation

Label-free NIR reflectance imaging as a complimentary tool for two-photon fluorescence microscopy: multimodal investigation of stroke (Conference Presentation)

Anna Letizia Allegra Mascaro, Irene Costantini, Emilia Margoni, Giulio Iannello, Alessandro Bria, Leonardo Sacconi, Francesco Pavone

Proceedings Volume 9712, 97121K (2016) https://doi.org/10.1117/12.2208886

KEYWORDS: Near infrared, Reflectivity, Brain, Two photon excitation microscopy, Two photon imaging, Photons, Axons, Blood circulation, Luminescence, Imaging spectroscopy

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Proceedings Article | 26 April 2016 Presentation

Mapping whole-brain activity with cellular resolution by light-sheet microscopy and high-throughput image analysis (Conference Presentation)

Ludovico Silvestri, Nikita Rudinskiy, Marco Paciscopi, Marie Caroline Müllenbroich, Irene Costantini, Leonardo Sacconi, Paolo Frasconi, Bradley Hyman, Francesco Pavone

Proceedings Volume 9690, 969012 (2016) https://doi.org/10.1117/12.2208603

KEYWORDS: Brain, Brain mapping, Image resolution, Microscopy, Image analysis, Image processing, Neurons, Imaging spectroscopy, Spectroscopy, Neuroimaging

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SPIE Journal Paper | 28 March 2016 Open Access

Clearing of fixed tissue: a review from a microscopist’s perspective

Ludovico Silvestri, Irene Costantini, Leonardo Sacconi, Francesco Saverio Pavone

JBO, Vol. 21, Issue 08, 081205, (March 2016) https://doi.org/10.1117/12.10.1117/1.JBO.21.8.081205

KEYWORDS: Tissues, Tissue optics, Luminescence, Brain, Refractive index, Microscopy, Transparency, Confocal microscopy, Taxonomy, Proteins

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Proceedings Article | 14 July 2015 Paper

A versatile new technique to clear mouse and human brain

Irene Costantini, Antonino Paolo Di Giovanna, Anna Letizia Allegra Mascaro, Ludovico Silvestri, Marie Caroline Müllenbroich, Leonardo Sacconi, Francesco Pavone

Proceedings Volume 9536, 95360X (2015) https://doi.org/10.1117/12.2184289

KEYWORDS: Brain, Tissues, Refractive index, Luminescence, Neuroimaging, Optical clearing, Microscopy, Transparency, Tomography, Image resolution

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SPIE Journal Paper | 29 June 2015 Open Access

Comprehensive optical and data management infrastructure for high-throughput light-sheet microscopy of whole mouse brains

M. Caroline Müllenbroich, Ludovico Silvestri, Leonardo Onofri, Irene Costantini, Marcel van’t Hoff, Leonardo Sacconi, Giulio Iannello, Francesco S. Pavone

NPh, Vol. 2, Issue 04, 041404, (June 2015) https://doi.org/10.1117/12.10.1117/1.NPh.2.4.041404

KEYWORDS: Brain, Microscopes, Cameras, Objectives, Neuroimaging, Mirrors, Microscopy, Confocal microscopy, Data acquisition, Camera shutters

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Proceedings Article | 10 March 2015 Paper

Whole brain optical imaging

Ludovico Silvestri, Anna Letizia Allegra Mascaro, Irene Costantini, Leonardo Sacconi, Francesco Pavone

Proceedings Volume 9305, 93051O (2015) https://doi.org/10.1117/12.2087339

KEYWORDS: Microscopy, Brain, Neuroimaging, Neurons, In vivo imaging, Electron microscopy, Axons, Optical imaging, Neurophotonics, Visualization

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Proceedings Article | 10 March 2015 Paper

A new versatile clearing method for brain imaging

Irene Costantini, Antonino Paolo Di Giovanna, Anna Letizia Allegra Mascaro, Ludovico Silvestri, Marie Caroline Müllenbroich, Leonardo Sacconi, Francesco Pavone

Proceedings Volume 9305, 930514 (2015) https://doi.org/10.1117/12.2076908

KEYWORDS: Brain, Tissues, Refractive index, Luminescence, Neuroimaging, Tissue optics, Optical clearing, Tomography, Microscopy, Brain imaging

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Proceedings Article | 5 March 2015 Open Access

Multiphoton microscopy in brain imaging

A. L. Allegra Mascaro, L. Silvestri, I. Costantini, L. Sacconi, B. Maco, G. Knott, F. Pavone

Proceedings Volume 9329, 932903 (2015) https://doi.org/10.1117/12.2084183

KEYWORDS: Neurons, Brain, Microscopy, In vivo imaging, Multiphoton microscopy, Two photon excitation microscopy, Brain imaging, Neuroimaging, Tissues, Confocal microscopy

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Proceedings Article | 28 February 2014 Paper

Exploring the brain on multiple scales with correlative two-photon and light sheet microscopy

Ludovico Silvestri, Anna Letizia Allegra Mascaro, Irene Costantini, Leonardo Sacconi, Francesco Pavone

Proceedings Volume 8948, 89480I (2014) https://doi.org/10.1117/12.2037867

KEYWORDS: Brain, Microscopy, Neurons, Blood vessels, In vivo imaging, Objectives, Neuroimaging, Two photon excitation microscopy, Confocal microscopy, Microscopes

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Proceedings Article | 17 June 2013 Paper

Neural plasticity explored by correlative two-photon and electron/SPIM microscopy

A. L. Allegra Mascaro, L. Silvestri, I. Costantini, L. Sacconi, B. Maco, G. Knott, F. Pavone

Proceedings Volume 8797, 87970G (2013) https://doi.org/10.1117/12.2032505

KEYWORDS: Microscopy, In vivo imaging, Neurons, Brain, Confocal microscopy, Neuroimaging, Electron microscopy, Axons, Two photon excitation microscopy, Luminescence

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Showing 5 of 23 publications

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