Prof. Jerry L. Chen Profile

Prof. Jerry L. Chen

at Boston Univ

SPIE Involvement:

Editorial Board Member: Neurophotonics | Author

Publications (5)

SPIE Journal Paper | 29 October 2024 Open Access

DeepVID v2: self-supervised denoising with decoupled spatiotemporal enhancement for low-photon voltage imaging

Chang Liu, Jiayu Lu, Yicun Wu, Xin Ye, Allison Ahrens, Jelena Platisa, Vincent Pieribone, Jerry Chen, Lei Tian

NPh, Vol. 11, Issue 04, 045007, (October 2024) https://doi.org/10.1117/12.10.1117/1.NPh.11.4.045007

KEYWORDS: Denoising, Signal to noise ratio, Video, Spatial resolution, Neurophotonics, Education and training, Spatial learning, Performance modeling, Data modeling, Neuroimaging

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SignificanceVoltage imaging is a powerful tool for studying the dynamics of neuronal activities in the brain. However, voltage imaging data are fundamentally corrupted by severe Poisson noise in the low-photon regime, which hinders the accurate extraction of neuronal activities. Self-supervised deep learning denoising methods have shown great potential in addressing the challenges in low-photon voltage imaging without the need for ground-truth but usually suffer from the trade-off between spatial and temporal performances.AimWe present DeepVID v2, a self-supervised denoising framework with decoupled spatial and temporal enhancement capability to significantly augment low-photon voltage imaging.ApproachDeepVID v2 is built on our original DeepVID framework, which performs frame-based denoising by utilizing a sequence of frames around the central frame targeted for denoising to leverage temporal information and ensure consistency. Similar to DeepVID, the network further integrates multiple blind pixels in the central frame to enrich the learning of local spatial information. In addition, DeepVID v2 introduces a new spatial prior extraction branch to capture fine structural details to learn high spatial resolution information. Two variants of DeepVID v2 are introduced to meet specific denoising needs: an online version tailored for real-time inference with a limited number of frames and an offline version designed to leverage the full dataset, achieving optimal temporal and spatial performances.ResultsWe demonstrate that DeepVID v2 is able to overcome the trade-off between spatial and temporal performances and achieve superior denoising capability in resolving both high-resolution spatial structures and rapid temporal neuronal activities. We further show that DeepVID v2 can generalize to different imaging conditions, including time-series measurements with various signal-to-noise ratios and extreme low-photon conditions.ConclusionsOur results underscore DeepVID v2 as a promising tool for enhancing voltage imaging. This framework has the potential to generalize to other low-photon imaging modalities and greatly facilitate the study of neuronal activities in the brain.

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Proceedings Article | 13 March 2024 Presentation

Resolution-improved self-supervised two-photon voltage imaging denoising

Chang Liu, Jelena Platisa, Xin Ye, Allison Ahrens, Ichun Anderson Chen, Ian Davison, Vincent Pieribone, Jerry Chen, Lei Tian

Proceedings Volume PC12828, PC1282807 (2024) https://doi.org/10.1117/12.3003148

KEYWORDS: Denoising, Two photon imaging, Spatial resolution, Signal to noise ratio, Visualization, Temporal resolution, Neurons, Image quality

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Proceedings Article | 16 March 2023 Presentation

Advancing low flux in vivo two-photon voltage imaging (Conference Presentation)

Jerry Chen

Proceedings Volume PC12390, PC1239007 (2023) https://doi.org/10.1117/12.2656201

KEYWORDS: In vivo imaging, Two photon imaging, Neurons, Microscopes, Imaging systems, Ultrafast phenomena, High speed imaging, Fluorescence spectroscopy, Denoising

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Proceedings Article | 15 March 2023 Presentation + Paper

Two-photon voltage imaging denoising by self-supervised learning

Chang Liu, Jelena Platisa, Xin Ye, Allison Ahrens, Ichun Anderson Chen, Ian Davison, Vincent Pieribone, Jerry Chen, Lei Tian

Proceedings Volume 12365, 1236505 (2023) https://doi.org/10.1117/12.2648122

KEYWORDS: Denoising, Two photon imaging, Fluorescence, Biological imaging, Signal to noise ratio, Neurons, Network architectures, Imaging systems, Deep learning, Machine learning, High speed imaging, Low light level imaging, Neural networks

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Proceedings Article | 5 March 2015 Paper

A modular two-photon microscope for simultaneous imaging of distant cortical areas in vivo

Fabian Voigt, Jerry Chen, Roland Krueppel, Fritjof Helmchen

Proceedings Volume 9329, 93292C (2015) https://doi.org/10.1117/12.2076388

KEYWORDS: Microscopes, Mirrors, Calcium, Scanners, In vivo imaging, Two photon imaging, Brain, Imaging systems, Pulsed laser operation, Relays

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