Dr. Jie Hui Profile

Dr. Jie Hui

Postdoctoral Fellow at Harvard Medical School

SPIE Involvement:

Editorial Board Member: Biophotonics Discovery | Author | Career Lab Leader

Area of Expertise:

Optical imaging , Antimicrobial phototherapy , Photoacoustic imaging , Microbiology , Photobiomodulation , Translational research

Publications (11)

Proceedings Article | 13 March 2024 Presentation

Translational development of antimicrobial blue light for wound infection control: new therapeutic window, treatment regimen, and wearable device

Jie Hui, Wonjoon Moon, Pu-Ting Dong, Carolina dos Anjos, Laisa Negri, Hao Yan, Ying Wang, Joshua Tam, Tianhong Dai, Rox Anderson, Jeremy Goverman, Jeff Gelfand, Seok-Hyun Andy Yun

Proceedings Volume PC12822, PC128220I (2024) https://doi.org/10.1117/12.3001119

KEYWORDS: Skin, Control systems, In vivo imaging, Wound healing, Thermal effects, Resistance, Light sources and illumination, Biomedical applications, Windows, Wearable devices

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Proceedings Article | 7 March 2022 Poster + Presentation

Photoinactivation of catalase sensitizes Candida albicans and Candida auris to ROS-producing agents and immune cells

Pu-Ting Dong, Yuewei Zhan, Sebastian Jusuf, Jie Hui, Zeina Dagher, Michael Mansour, Ji-Xin Cheng

Proceedings Volume PC11940, (2022) https://doi.org/10.1117/12.2609218

KEYWORDS: Resistance, Fungi, Standards development, Skin, Proteins, Pathogens, Oxygen, Organisms, Mouse models, Medicine

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Proceedings Article | 10 March 2020 Presentation

Eradication of broad-spectrum multi-drug fungal pathogens through photoinactivation of a detoxifying enzyme (Conference Presentation)

Pu-Ting Dong, Zeina Dagher, Yuewei Zhan, Jie Hui, Michael Mansour, Ji-Xin Cheng

Proceedings Volume 11223, 112230U (2020) https://doi.org/10.1117/12.2546838

KEYWORDS: Pathogens, Hydrogen, Lung, Oxygen

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Proceedings Article | 10 March 2020 Presentation

Imaging Amphotericin B orientation in Candida spp. through polarization stimulated Raman scattering microscopy (Conference Presentation)

Pu-Ting Dong, Yuewei Zhan, Zeina Dagher, Jie Hui, Kai-Chih Huang, Michael Mansour, Ji-Xin Cheng

Proceedings Volume 11252, 1125207 (2020) https://doi.org/10.1117/12.2546819

KEYWORDS: Amplifiers, Polarization, Raman scattering, Microscopy, Resistance, Defense and security, Circular dichroism spectroscopy, Atomic force microscopy, Fluorescence spectroscopy, Visualization

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Proceedings Article | 19 February 2020 Presentation + Paper

Inhibiting staphylococcus aureus antibiotic resistance via photo-disassembly of membrane microdomains

Jie Hui, Pu-Ting Dong, Lijia Liang, Taraknath Mandal, Junjie Li, Erlinda Ulloa, Yuewei Zhan, Sebastian Jusuf, Cheng Zong, Mohamed Seleem, George Liu, Qiang Cui, Ji-Xin Cheng

Proceedings Volume 11223, 112230T (2020) https://doi.org/10.1117/12.2543045

KEYWORDS: Resistance, Laser therapeutics, Photolysis, Pulsed laser operation, Raman spectroscopy, Luminescence, Proteins, Phototherapy, Light emitting diodes, Statistical analysis

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Proceedings Article | 13 March 2019 Presentation

Eradication of broad-spectrum intracellular bacteria through photoinactivation of a detoxifying enzyme (Conference Presentation)

Pu-Ting Dong, Jie Hui, Junjie Li, Yifan Zhu, Lijia Liang, Ji-Xin Cheng

Proceedings Volume 10863, 1086313 (2019) https://doi.org/10.1117/12.2508693

KEYWORDS: Bacteria, Defense and security, Pathogens, Resistance, Optical imaging, Visualization

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Proceedings Article | 4 March 2019 Presentation

Photo-disassembly of membrane microdomains revives a broad spectrum of antibiotics against MRSA persisters (Conference Presentation)

Jie Hui, Pu-Ting Dong, Junjie Li, Lijia Liang, Cheng Zong, Ji-Xin Cheng

Proceedings Volume 10863, 108630V (2019) https://doi.org/10.1117/12.2509497

KEYWORDS: Resistance, Proteins, Cell mechanics, Pulsed laser operation, Laser safety, Safety, Light sources, Raman spectroscopy, Photolysis, Luminescence

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The prevalence of antibiotic resistance and the presence of bacterial persisters increasingly challenge the successful treatment of Staphylococcus aureus infections, and thus poses a great threat to the global health. Here, we present a photonic approach to revive a broad spectrum of antibiotics for eradication of MRSA persisters via photo-disassembly of functional membrane microdomains. Membrane microdomains on MRSA cells are enriched in staphyloxanthin-derived lipids as constituent lipids with co-localized and oligomerized multimeric protein complexes including PBP2a to execute various cellular processes and cell virulence. We demonstrated that the membrane-bound staphyloxinthin is prone to photobleaching by blue light due to triplet-triplet annihilation and thus compromises the membrane integrity. Using high-intensity 460 nm pulsed laser (wide-field illumination, dosage far below human safety limit), we achieved strikingly high staphyloxanthin bleaching efficiency and depth when compared to low-level light sources (quantified by resonance Raman spectroscopy). More importantly, such efficient and selective photolysis of constituent lipids leads to catastrophic disassembly of membrane microdomains, yielding highly compromised cell membrane with nanometer-scale pores created and PBP2a unanchored from cell membrane or dispersed (proved and quantified by immunofluorescence, fluorescence assay, confocal, super-resolution imaging, and Western blotting). The disruption renders MRSA persisters highly traumatized, thus no longer in dormant state (verified by stimulated Raman scattering microscopy). Consequently, cells with compromised membrane are found highly susceptible to a broad spectrum of antibiotics: beta-lactam antibiotics, such as penicillin and cephalosporins, due to PBP2a disassembly; antibiotics that inhibit intracellular activities enabled by effective diffusion via nanometer-scale pores, such as quinolones, aminoglycosides and sulfonamides. These synergistic therapies are validated both in vitro and in clinically relative models including biofilm and mice skin infection model. Collective, our findings unveil the underlying mechanism of photo-disassembly of MRSA membrane microdomains and highlight this photonic approach as a novel platform to revive a broad spectrum of conventional antibiotics and guide the development of new antibiotics for treatment of MRSA infections.

Proceedings Article | 8 February 2018 Presentation + Paper

Staphyloxanthin photobleaching sensitizes methicillin-resistant Staphylococcus aureus to reactive oxygen species attack

Pu-Ting Dong, Haroon Mohammad, Jie Hui, Xiaoyu Wang, Junjie Li, Lijia Liang, Mohamed Seleem, Ji-Xin Cheng

Proceedings Volume 10479, 104790R (2018) https://doi.org/10.1117/12.2291248

KEYWORDS: Absorption, Oxygen, Photonics, Mass spectrometry, Chromophores, Resistance, Hydrogen, Error analysis, Microscopes, Light emitting diodes, Microbiology, Biophysics, Photomedicine, Antimicrobial agents

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Proceedings Article | 3 March 2017 Paper

Real-time intravascular photoacoustic-ultrasound imaging of lipid-laden plaque at speed of video-rate level

Jie Hui, Yingchun Cao, Yi Zhang, Ayeeshik Kole, Pu Wang, Guangli Yu, Gregory Eakins, Michael Sturek, Weibiao Chen, Ji-Xin Cheng

Proceedings Volume 10064, 100640T (2017) https://doi.org/10.1117/12.2251511

KEYWORDS: Image resolution, In vivo imaging, Fiber optics, Ultrasonography, Mirrors, Imaging systems, Real time imaging, Photoacoustic imaging, Absorption, Image processing, High speed imaging

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Proceedings Article | 27 April 2016 Presentation

High-speed intravascular photoacoustic imaging of lipid-laden plaque at 1.7 micron (Conference Presentation)

Jie Hui, Qianhuan Yu, Teng Ma, Pu Wang, Yingchun Cao, Rebecca Bruning, Yueqiao Qu, Zhongping Chen, Qifa Zhou, Michael Sturek, Weibiao Chen, Ji-Xin Cheng

Proceedings Volume 9708, 970804 (2016) https://doi.org/10.1117/12.2212200

KEYWORDS: Imaging systems, Optical parametric oscillators, Photoacoustic imaging, Mechanics, Photoacoustic spectroscopy, High power lasers, Laser sources, Potassium, Lead, Image resolution

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Proceedings Article | 11 March 2015 Paper

Assessing carotid atherosclerosis by fiber-optic multispectral photoacoustic tomography

Jie Hui, Rui Li, Pu Wang, Evan Phillips, Rebecca Bruning, Chien-Sheng Liao, Michael Sturek, Craig Goergen, Ji-Xin Cheng

Proceedings Volume 9323, 93233S (2015) https://doi.org/10.1117/12.2076999

KEYWORDS: Ultrasonography, Arteries, Fiber optics, Multispectral imaging, Blood, Photoacoustic tomography, Chemical analysis, Imaging systems, Acquisition tracking and pointing, Breast

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Showing 5 of 11 publications

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