We report a system for optical trapping, manipulation and imaging of live cells in
close to real-time with a wide field of view.
A fs-pulsed Ti:Sapphire source was used to trap cells whilst an Ar+ laser was used
for confocal imaging. A single 10x/0.4NA lens and a dual scanning system enabled
independent trapping, imaging and movement of cells over a field of view >1mm2.
We will present movies of controlled T cell and dendritic cell movement and
interaction. This enables the functional study of cell-signalling processes that are
fundamental to understanding the behaviour of the immune system and beyond.