KEYWORDS: Neurons, Optogenetics, Brain, In vivo imaging, In vitro testing, Cerebellum, Thalamus, Information operations, Electromyography, Analog electronics
We employed a transgenic mouse having conditional expression of ChR2(H134R) in neurons of the inferior olive to
facilitate understanding of the role of electrical coupling and oscillation in central nervous system function. Two-photon
excitation of ChR2-expressing neurons using 64 laser beams restricted to single inferior olive cell bodies depolarized
neurons and evoked voltage deflections in neighboring neurons demonstrating electrical coupling. Broader illumination
of neuronal ensembles using blue light induced an optical clamp of endogenous electrical rhythms in the inferior olive of
acutely-prepared brain slices, which when applied in vivo directly modulated the local field potential activity and
induced tremor. The experiments demonstrate novel methods to optically manipulate electrically coupled potentials and
rhythmogenesis within a neuronal ensemble. From a functional perspective, the experiments shed light on the cellular
and circuitry mechanisms of essential tremor, a prevalent neurological condition, by indicating time- and frequencydependence
of tremor upon varying rhythms of inferior olive stimulation. The experiments indicate analog control of a
brain rhythm that may be used to enhance our understanding of the functional consequences of central rhythmogenesis.
Conference Committee Involvement (11)
Optogenetics and Optical Manipulation 2023
28 January 2023 | San Francisco, California, United States
Optogenetics and Optical Manipulation 2022
22 January 2022 | San Francisco, California, United States
Optogenetics and Optical Manipulation 2021
6 March 2021 | Online Only, California, United States
Optogenetics and Optical Manipulation 2020
1 February 2020 | San Francisco, California, United States
Optogenetics and Optical Manipulation 2019
2 February 2019 | San Francisco, California, United States
Optogenetics and Optical Manipulation 2018
27 January 2018 | San Francisco, California, United States
Optogenetics and Optical Manipulation
28 January 2017 | San Francisco, California, United States
Optogenetics and Optical Manipulation
13 February 2016 | San Francisco, California, United States
Optogenetics and Optical Control of Cells
7 February 2015 | San Francisco, California, United States
Optogenetics and Optical Control of Cells
1 February 2014 | San Francisco, California, United States
Optogenetics and Hybrid-Optical Control of Cells
2 February 2013 | San Francisco, California, United States
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