We present a deep label-free quantitative phase imaging method using confocal gradient light interference microscopy (CGLIM) for studying ex vivo three-dimensional cellular clusters and in vivo mouse brains. CGLIM is a custom-built confocal reflectance differential interference contrast (DIC) microscope with phase-shifting interferometry using long-wavelength (~1.7 μm) illumination. As a laser-scanning common-path interferometric technique based on linear scattering, CGLIM has superior stability and sensitivity in phase measurement and reduces photodamage, or heating to living systems. With the proposed method, we will demonstrate the working principle on standard samples, and the phase imaging of cancer spheroids and in vivo mouse brains.
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