Fluorescent gold nanoclusters (AuNCs) with emission in near infrared (NIR) range have drawn wide attention due to its bright fluorescence, good biocompatibility and photostability. In this work, AuNCs with emission at 800 nm were synthesized through a simple glutathione reduction method through heating. Cyclic RGD peptide will be conjugated through thiol-PEG-amine as targeting ligand. The AuNCs are then used for two photon fluorescent imaging, due to the wide stokes shift. In vivo NIR fluorescent study showed the accumulation of the AuNCs in tumor areas. Moreover, the AuNCs also showed brain uptake, which indicates possible applications in brain imaging.
Fluorescence guided surgery has high potential for improved patient outcomes but often lacks quantification of fluorophore depth which is needed to determine surgical margins of solid tumors. To address this need, a dual wavelength excitation approach was applied that capitalizes on the wavelength-dependent attenuation of light in tissue to provide depth information independent of fluorophore concentration. A portable near infrared dual wavelength excitation fluorescence imaging system was built and tested using tissue mimicking phantoms and is currently being tested to determine breast tumor margin status in a first-in-human clinical trial investigating LS301, a novel near infrared tumor-targeted contrast agent.
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