Quantification of fibrosis is critical for the management of inflammatory bowel disease. In this study, photoacoustic (PA) strain imaging were used to estimate intestinal stiffness during the progression of intestinal fibrosis in 23 rabbits in vivo. The tissue was then harvested to measure the young’s modulus ex vivo. Collagen-to-Hb ratio measured using spectroscopic PA imaging was also recorded. Results show that PA-strain is positively correlated to Young’s Modulus with a correlation coefficient of 0.81. PA-strain distinguishes the low histological fibrosis (0-2) and high histological fibrosis (3-5) significantly (p-value<0.001). Collagen-to-Hb ratio and PA-strain are highly correlated with the histological fibrosis (0-5) with correlation of 0.67 and 0.64, respectively.
Quantification of fibrosis is critical for the management of inflammatory bowel disease. In this study, two measurements, collagen-to-Hb ratio quantified by spectroscopic analysis and tissue stiffness quantified by PA-strain, measured by our PA-US balloon catheter were employed to quantify intestinal fibrosis in 23 rabbits in vivo. Results show that both measurements can distinguish the low histological fibrosis (0-2) and high histological fibrosis (3-5) with statistical significance (p-value<0.001). Collagen-to-Hb ratio and PA-strain are highly correlated with the fibrosis stages with correlation of 0.67 and 0.64, respectively. PA-strain is positively correlated to Young’s Modulus measured ex vivo using microelastometer with correlation 0.81.
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