Photoacoustic microscopy (PAM) allows for the visualization of microscale structures and functionalities by detecting various chromophores in biological tissues based on their absorption spectra at specific laser wavelengths. PAM differentiates oxy and deoxy blood, water, collagen, and lipid with unique absorption spectra in NIR. To enhance the functionality of PAM, integrating multi-wavelength laser sources, particularly Ti:Sapphire lasers, is gaining significant attention. Ti:Sapphire lasers are considered an advanced solution due to their high energy efficiency and wavelength tunability. This presentation introduces a single-shot, wavelength-tunable Ti:Sapphire-based multispectral PAM system capable of rapidly performing functional imaging of blood concentrations and ICG-lymphography.
Photoacoustic imaging technology using multi-wavelength can achieve functional image that provide information about a singularity in an image target. We developed a wavelength tunable light source pumped by few tens kHz pulsed laser with 532 nm wavelength for single scan functional photoacoustic imaging. The laser has individual output pulses with about 200 nsec time delay between the original 532 nm pump pulse and the wavelength tunable pulse. By using the ping-pong pulse output, we have make enable to obtain a functional photoacoustic image in a single laser scanning and demonstrated functional photoacoustic microscopy imaging to show a mouse activity.
Optical resolution-photoacoustic microscopy (OR-PAM) is a microscopic system to provide optical absorption contrast in biological tissue. Currently, high-speed OR-PAM scanning faced the limitation of the millimeter-scale field of view (FOV). Without hardware updates, mosaic processing was used to generate a wide FOV PAM image by merging narrow-ranged PAM images. Using feature generation algorithms, feature points were selected by binary decision and were merged by homography estimation. In this study, the diverse feature generation algorithms were applied and compared to estimate their performances for mosaic PAM imaging. Based on the results, mosaic processing implemented with a wide-field OR-PAM system was applied.
Photoacoustic microscopy (PAM) is a multiscale microscopy technique with optical absorption contrast. With tight acoustic focusing, acoustic-resolution (AR-PAM) reaches the depth of several centimeters in biological tissue, but the lateral resolution is relatively poorer than optical-resolution PAM (OR-PAM). We proposed using CycleGAN to generate new OR-PAM images from the original AR-PAM images. We prepared two AR & OR-PAM datasets from leaf phantom and mouse-ear samples. After completing the CycleGAN process, we estimated the quality comparison between the original AR-PAM and the generated OR-PAM images. Finally, the results showed the ability to obtain high spatial resolution PAM images without hardware updates.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.