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Li Juan

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Proceedings Article | 6 March 2009 Paper

Quantity analysis of micro-organisms in bottled water

Juan Li, Xiangyong Li

Proceedings Volume 7280, 72800U (2009) https://doi.org/10.1117/12.821601

KEYWORDS: Water, Microorganisms, Bacteria, Microscopes, Luminescence, Optical filters, Viruses, Photomicroscopy, Analytical research, Biological research

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Water is necessary to human being and all kinds of animals and plants. In recently years, Bottled Water become the main drinking water whatever for families or for institutions. But most of them have no conception of the water's safety or quality. To have conceptions of the count and distributing of the microorganisms in bucket pure water, we use fluorescent microscope counting stained with SYBR Green I to research the microorganisms (including virus) quantity in Bottled Water for six samples produced in different place. Analyzing shows that the quantity of the microorganisms in these water are different. Some up to 11.912×10⁶ virus/ m L. The quality of Bottled Water needs to be improved. And the quantity of microorganisms in the water is different with different ways to keep the water. At the same time, it shows that fluorescent microscope counting stained with SYBR Green I method is simple and high sensitive to such low microorganisms quantity water sample. It can be used in the microorganisms dynamic quantity research in drinking water.

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