Dr. Lijia Liang Profile

Dr. Lijia Liang

at Jilin Univ

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Author

Publications (4)

Proceedings Article | 19 February 2020 Presentation + Paper

Inhibiting staphylococcus aureus antibiotic resistance via photo-disassembly of membrane microdomains

Jie Hui, Pu-Ting Dong, Lijia Liang, Taraknath Mandal, Junjie Li, Erlinda Ulloa, Yuewei Zhan, Sebastian Jusuf, Cheng Zong, Mohamed Seleem, George Liu, Qiang Cui, Ji-Xin Cheng

Proceedings Volume 11223, 112230T (2020) https://doi.org/10.1117/12.2543045

KEYWORDS: Resistance, Laser therapeutics, Photolysis, Pulsed laser operation, Raman spectroscopy, Luminescence, Proteins, Phototherapy, Light emitting diodes, Statistical analysis

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Proceedings Article | 13 March 2019 Presentation

Eradication of broad-spectrum intracellular bacteria through photoinactivation of a detoxifying enzyme (Conference Presentation)

Pu-Ting Dong, Jie Hui, Junjie Li, Yifan Zhu, Lijia Liang, Ji-Xin Cheng

Proceedings Volume 10863, 1086313 (2019) https://doi.org/10.1117/12.2508693

KEYWORDS: Bacteria, Defense and security, Pathogens, Resistance, Optical imaging, Visualization

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Proceedings Article | 4 March 2019 Presentation

Photo-disassembly of membrane microdomains revives a broad spectrum of antibiotics against MRSA persisters (Conference Presentation)

Jie Hui, Pu-Ting Dong, Junjie Li, Lijia Liang, Cheng Zong, Ji-Xin Cheng

Proceedings Volume 10863, 108630V (2019) https://doi.org/10.1117/12.2509497

KEYWORDS: Resistance, Proteins, Cell mechanics, Pulsed laser operation, Laser safety, Safety, Light sources, Raman spectroscopy, Photolysis, Luminescence

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The prevalence of antibiotic resistance and the presence of bacterial persisters increasingly challenge the successful treatment of Staphylococcus aureus infections, and thus poses a great threat to the global health. Here, we present a photonic approach to revive a broad spectrum of antibiotics for eradication of MRSA persisters via photo-disassembly of functional membrane microdomains. Membrane microdomains on MRSA cells are enriched in staphyloxanthin-derived lipids as constituent lipids with co-localized and oligomerized multimeric protein complexes including PBP2a to execute various cellular processes and cell virulence. We demonstrated that the membrane-bound staphyloxinthin is prone to photobleaching by blue light due to triplet-triplet annihilation and thus compromises the membrane integrity. Using high-intensity 460 nm pulsed laser (wide-field illumination, dosage far below human safety limit), we achieved strikingly high staphyloxanthin bleaching efficiency and depth when compared to low-level light sources (quantified by resonance Raman spectroscopy). More importantly, such efficient and selective photolysis of constituent lipids leads to catastrophic disassembly of membrane microdomains, yielding highly compromised cell membrane with nanometer-scale pores created and PBP2a unanchored from cell membrane or dispersed (proved and quantified by immunofluorescence, fluorescence assay, confocal, super-resolution imaging, and Western blotting). The disruption renders MRSA persisters highly traumatized, thus no longer in dormant state (verified by stimulated Raman scattering microscopy). Consequently, cells with compromised membrane are found highly susceptible to a broad spectrum of antibiotics: beta-lactam antibiotics, such as penicillin and cephalosporins, due to PBP2a disassembly; antibiotics that inhibit intracellular activities enabled by effective diffusion via nanometer-scale pores, such as quinolones, aminoglycosides and sulfonamides. These synergistic therapies are validated both in vitro and in clinically relative models including biofilm and mice skin infection model. Collective, our findings unveil the underlying mechanism of photo-disassembly of MRSA membrane microdomains and highlight this photonic approach as a novel platform to revive a broad spectrum of conventional antibiotics and guide the development of new antibiotics for treatment of MRSA infections.

Proceedings Article | 8 February 2018 Presentation + Paper

Staphyloxanthin photobleaching sensitizes methicillin-resistant Staphylococcus aureus to reactive oxygen species attack

Pu-Ting Dong, Haroon Mohammad, Jie Hui, Xiaoyu Wang, Junjie Li, Lijia Liang, Mohamed Seleem, Ji-Xin Cheng

Proceedings Volume 10479, 104790R (2018) https://doi.org/10.1117/12.2291248

KEYWORDS: Absorption, Oxygen, Photonics, Mass spectrometry, Chromophores, Resistance, Hydrogen, Error analysis, Microscopes, Light emitting diodes, Microbiology, Biophysics, Photomedicine, Antimicrobial agents

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