The absorption spectrum and fluorescence spectrum of thylakoid(sample I) and chlorophyll (sample II) extracted
from brassica chinensis were studied based on polarization technique. As a result, the absorption peak positions of
sample I red shift by more than ten nanometers comparing sample II and the absorption intensities of sample I
declined when the polarizer went round from 0° to 90°. It gave detailed explanation why the two absorption spectra
feel so different. On the other side, the polarization fluorescence spectra of the two samples excited by 437nm were
investigated respectively and the calculation of the fluorescence polarization degree which is determined by the
environment of the pigment, the bound state, the effectual energy transfer and the ordered arrangement of the
pigment in the two samples showed that the pigment in sample I arrayed regularily. The results of the study would
provide powerful reference to the research of the energy transfer and transformation during photosynthesis.
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