Ms. Liubov E. Shimolina Profile

Liubov E. Shimolina

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Publications (9)

SPIE Journal Paper | 30 September 2024 Open Access

Simultaneous assessment of NAD(P)H and flavins with multispectral fluorescence lifetime imaging microscopy at a single excitation wavelength of 750 nm

Boris Yakimov, Anastasia Komarova, Elena Nikonova, Artem Mozherov, Liubov Shimolina, Marina Shirmanova, Wolfgang Becker, Evgeny Shirshin, Vladislav Shcheslavskiy

JBO, Vol. 29, Issue 10, 106501, (September 2024) https://doi.org/10.1117/12.10.1117/1.JBO.29.10.106501

KEYWORDS: Fluorescence, Fluorescence lifetime imaging, Nicotinamide adenine dinucleotide, Emission wavelengths, Mode conditioning cables, Sensors, Fluorescence intensity, Cancer, Microscopy, Tumors

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SignificanceAutofluorescence characteristics of the reduced nicotinamide adenine dinucleotide and oxidized flavin cofactors are important for the evaluation of the metabolic status of the cells. The approaches that involve a detailed analysis of both spectral and time characteristics of the autofluorescence signals may provide additional insights into the biochemical processes in the cells and biological tissues and facilitate the transition of spectral fluorescence lifetime imaging into clinical applications.AimWe present the experiments on multispectral fluorescence lifetime imaging with a detailed analysis of the fluorescence decays and spectral profiles of the reduced nicotinamide adenine dinucleotide and oxidized flavin under a single excitation wavelength aimed at understanding whether the use of multispectral detection is helpful for metabolic imaging of cancer cells.ApproachWe use two-photon spectral fluorescence lifetime imaging microscopy. Starting from model solutions, we switched to cell cultures treated by metabolic inhibitors and then studied the metabolism of cells within tumor spheroids.ResultsThe use of a multispectral detector in combination with an excitation at a single wavelength of 750 nm allows the identification of fluorescence signals from three components: free and bound NAD(P)H, and flavins based on the global fitting procedure. Multispectral data make it possible to assess not only the lifetime but also the spectral shifts of emission of flavins caused by chemical perturbations. Altogether, the informative parameters of the developed approach are the ratio of free and bound NAD(P)H amplitudes, the decay time of bound NAD(P)H, the amplitude of flavin fluorescence signal, the fluorescence decay time of flavins, and the spectral shift of the emission signal of flavins. Hence, with multispectral fluorescence lifetime imaging, we get five independent parameters, of which three are related to flavins.ConclusionsThe approach to probe the metabolic state of cells in culture and spheroids using excitation at a single wavelength of 750 nm and a fluorescence time-resolved spectral detection with the consequent global analysis of the data not only simplifies image acquisition protocol but also allows to disentangle the impacts of free and bound NAD(P)H, and flavin components evaluate changes in their fluorescence parameters (emission spectra and fluorescence lifetime) upon treating cells with metabolic inhibitors and sense metabolic heterogeneity within 3D tumor spheroids.

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Proceedings Article | 18 June 2024 Presentation + Paper

Viscoelastic properties of human colorectal cancer cells with different migration activity

Liubov Shimolina, Yuri Efremov, Nadezhda Ignatova, Alexander Gulin, Irina Druzhkova, Vladislav Shcheslavskiy, Marina Kuimova, Peter Timashev, Marina Shirmanova

Proceedings Volume 13010, 1301008 (2024) https://doi.org/10.1117/12.3026232

KEYWORDS: Colorectal cancer, Cancer, Fluorescence lifetime imaging, Atomic force microscopy, Viscoelasticity, Tumors, Fluorescence

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Proceedings Article | 7 March 2022 Presentation

Involvement of the cytoplasmic membrane viscosity of tumor cells in response to chemotherapy with platinum-based drugs

Liubov Shimolina, Marina Kuimova, Maria Lukina, Nadezhda Ignatova, Irina Druzhkova, Marina Shirmanova

Proceedings Volume PC11965, PC119650J (2022) https://doi.org/10.1117/12.2606791

KEYWORDS: Tumors, Fluorescence lifetime imaging, Cancer, Colorectal cancer, Platinum, Plasma, Microscopes, Laser scanners, In vitro testing

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Proceedings Article | 5 March 2021 Presentation

Ex vivo metabolic imaging of tumor samples from patients by FLIM

Maria Lukina, Lubov Shimolina, Varvara Dudenkova, Alena Gavrina, Vladimir Zagainov, Dmitriy Komarov, Konstantin Yashin, Igor Medyanik, Elena Zagaynova, Marina Shirmanova

Proceedings Volume 11648, 116480N (2021) https://doi.org/10.1117/12.2579211

KEYWORDS: Tumors, Fluorescence lifetime imaging, Cancer, Mode conditioning cables, Luminescence, Colorectal cancer, Tissues, Tissue optics, Proteins

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SPIE Journal Paper | 16 December 2020 Open Access

Mapping cisplatin-induced viscosity alterations in cancer cells using molecular rotor and fluorescence lifetime imaging microscopy

Liubov Shimolina, Alexander Gulin, Miguel Paez-Perez, Ismael López-Duarte, Irina Druzhkova, Maria Lukina, Margarita Gubina, Nicolas Brooks, Elena Zagaynova, Marina Kuimova, Marina Shirmanova

JBO, Vol. 25, Issue 12, 126004, (December 2020) https://doi.org/10.1117/12.10.1117/1.JBO.25.12.126004

KEYWORDS: Plasma, Cancer, Fluorescence lifetime imaging, Luminescence, Tumors, Ions, Cell death, Resistance, Oncology, Microscopy

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Proceedings Article | 14 February 2020 Paper

Imaging plasma membrane microviscosity in cancer cells during chemotherapy

Liubov Shimolina, Marina Shirmanova, Marina Kuimova, Maria Lukina, Nadezhda Ignatova, Irina Druzhkova, Elena Zagaynova

Proceedings Volume 11244, 112442M (2020) https://doi.org/10.1117/12.2550133

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Cancer, Tumors, Microscopy, Resistance, Cell death

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Proceedings Article | 22 July 2019 Paper

Probing chemosensitivity and energy metabolism in patients-derived colorectal cancer cells

Irina Druzhkova, Maria Lukina, Nadezhda Ignatova, Varvara Dudenkova, Marina Shirmanova, Vladimir Zagainov, Nikolay Kiselev, Lyubov' Shimolina, Elena Zagaynova

Proceedings Volume 11076, 110761K (2019) https://doi.org/10.1117/12.2527021

KEYWORDS: Colorectal cancer, Fluorescence lifetime imaging, Cancer, Tumors, Luminescence, Mode conditioning cables, Oncology, Collagen, Medical research, Tissues

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Proceedings Article | 17 May 2018 Paper

Insight into microenvironment of tumor on the microscopic level with a focus on cancer-associated fibroblasts

Irina Druzhkova, Maria Lukina, Varvara Dudenkova, Lubov Shimolina, Elena Zagaynova, Marina Shirmanova

Proceedings Volume 10685, 106852R (2018) https://doi.org/10.1117/12.2307096

KEYWORDS: Collagen, Tumors, Luminescence, Cancer, Second-harmonic generation, Fluorescence lifetime imaging, Mode conditioning cables, 3D modeling, Skin, Microscopy

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Proceedings Article | 28 July 2017 Paper

Probing energy metabolism and microviscosity in cancer using FLIM

Marina Shirmanova, Maria Lukina, Lyubov’ Shimolina, Marina Kuimova, Varvara Dudenkova, Vladislav Shcheslavskiy, Elena Zagaynova

Proceedings Volume 10411, 1041102 (2017) https://doi.org/10.1117/12.2287094

KEYWORDS: Mode conditioning cables, Cancer, Fluorescence lifetime imaging, Microscopy

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Showing 5 of 9 publications

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