Hyperspectral images of the human iris, containing spectral and spatial information of the iris, are acquired by a custom developed instrument. We developed this instrument specifically to perform in-vivo spectral reflectance measurements of the human iris. The significance of the data cube acquired is strictly related to the acquisition system performance. This paper analyzes the qualitative and quantitative in-vivo performances of our instrument considering in-vivo repeatability tests and comparing left and right irises of normal subjects, without visually assessed heterochromia of the irises.
We describe an optical system and a method for measuring the human iris spectral reflectance in vivo by hyperspectral imaging analysis. It is important to monitor age-related changes in the reflectance properties of the iris as they are a prognostic factor for several eye pathologies. In this paper, we report the outcomes of our most recent research, resulting from the improvement of our imaging system. In particular, a custom tunable light source was developed: the images are now acquired in the spectral range 440 - 900 nm. With this system, we are able to obtain a spectral resolution of 20nm, while each image of 2048 x 1536 pixels has a spatial resolution of 10.7 μm. The results suggest that the instrument could be exploited for measuring iris pigmentation changes over time. These measurements could provide new diagnostic capabilities in ophthalmology. Further studies are required to determine the measurements’ repeatability and to develop a spectral library for results evaluation and to detect differences among subsequent screenings of the same subject.
In this paper we present a fiber-based low-coherence self-mixing interferometer exploiting a single-arm approach to measure the flow in a pipe. The main advantages of the proposed system are the flexibility offered by fiber-connected optical head, a greater ease of alignment, the rejection of “common-mode” vibrations, and greater stability. Thanks to the use of a low-coherence source, the proposed system investigates the velocity of the scattering particles owing only in a fixed and well defined region located close to the duct wall itself. The reported experimental results demonstrate that in laminar flow regime the developed system is able to determine the flow and it is quite robust to variation in the scatterers concentration. Increasing the scatterers concentration of about 24 times, the sensitivity S has reduced of less than 30%.
According to previous studies, the measurement of the human iris pigmentation can be exploited to detect certain eye pathological conditions in their early stage. In this paper, we propose an instrument and a method to perform hyperspectral quantitative measurements of the iris spectral reflectance. The system is based on a simple imaging setup, which includes a monochrome camera mounted on a standard ophthalmic microscope movement controller, a monochromator, and a flashing LED-based slit lamp. To assure quantitative measurements, the system is properly calibrated against a NIST reflectance standard. Iris reflectance images can be obtained in the spectral range 495-795 nm with a resolution of 25 nm. Each image consists of 1280 x 1024 pixels having a spatial resolution of 18 μm. Reflectance spectra can be calculated both from discrete areas of the iris and as the average of the whole iris surface. Preliminary results suggest that hyperspectral imaging of the iris can provide much more morphological and spectral information with respect to conventional qualitative colorimetric methods.
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