Dr. Margaux Bouzin Profile

Dr. Margaux Bouzin

Post-doctoral researcher at Università degli Studi di Milano-Bicocca

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Publications (5)

SPIE Journal Paper | 27 February 2019

Adaptive optics microspectrometer for cross-correlation measurement of microfluidic flows

Maddalena Collini, Fabrizio Radaelli, Laura Sironi, Nicolo Ceffa, Laura D'Alfonso, Margaux Bouzin, Giuseppe Chirico

JBO Vol. 24 Issue 02

KEYWORDS: Spatial light modulators, Aberration correction, Adaptive optics, Cameras, Optical aberrations, Wavefronts, Microfluidics, Statistical analysis, Luminescence, Rhodamine

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Proceedings Article | 22 June 2015 Paper

Single image correlation for blood flow mapping in complex vessel networks

Giuseppe Chirico, Laura Sironi, Margaux Bouzin, Laura D'Alfonso, Maddalena Collini, Nicolo’ Ceffa, Cassia Marquezin

Proc. SPIE. 9529, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II

KEYWORDS: Confocal microscopy, Tissues, Capillaries, Luminescence, Diffusion, Image analysis, Blood circulation, Hemodynamics, Fluorescence correlation spectroscopy, Spatial resolution

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Microcirculation plays a key role in the maintenance and hemodynamics of tissues and organs also due to its extensive interaction with the immune system. A critical limitation of state-of-the-art clinical techniques to characterize the blood flow is their lack of the spatial resolution required to scale down to individual capillaries. On the other hand the study of the blood flow through auto- or cross-correlation methods fail to correlate the flow speed values with the morphological details required to describe an intricate network of capillaries. Here we propose to use a newly developed technique (FLICS, FLow Image Correlation Spectroscopy) that, by employing a single raster-scanned xy-image acquired in vivo by confocal or multi-photon excitation fluorescence microscopy, allows the quantitative measurement of the blood flow velocity in the whole vessel pattern within the field of view, while simultaneously maintaining the morphological information on the immobile structures of the explored circulatory system. Fluorescent flowing objects produce diagonal lines in the raster-scanned image superimposed to static morphological details. The flow velocity is obtained by computing the Cross Correlation Function (CCF) of the intensity fluctuations detected in pairs of columns of the image. The whole analytical dependence of the CCFs on the flow speed amplitude and the flow direction has been reported recently. We report here the derivation of approximated analytical relations that allows to use the CCF peak lag time and the corresponding CCF value, to directly estimate the flow speed amplitude and the flow direction. The validation has been performed on Zebrafish embryos for which the flow direction was changed systematically by rotating the embryos on the microscope stage. The results indicate that also from the CCF peak lag time it is possible to recover the flow speed amplitude within 13% of uncertainty (overestimation) in a wide range of angles between the flow and the image scanning direction.

Proceedings Article | 5 March 2015 Paper

Fluorescence cross-correlation spectroscopy for time dependent flows: a numerical investigation

Nicolo' Ceffa, Paolo Pozzi, Margaux Bouzin, Cassia Marquezin, Laura Sironi, Laura D'Alfonso, Maddalena Collini, Giuseppe Chirico

Proc. SPIE. 9320, Microfluidics, BioMEMS, and Medical Microsystems XIII

KEYWORDS: Microfluidics, Modulation, Blood, Capillaries, Spectroscopy, Luminescence, Diffusion, Fluorescence spectroscopy, Numerical analysis, Blood circulation

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SPIE Journal Paper | 20 June 2014

Electron multiplying charge-coupled device-based fluorescence cross-correlation spectroscopy for blood velocimetry on zebrafish embryos

Paolo Pozzi, Laura Sironi, Laura D’Alfonso, Margaux Bouzin, Maddalena Collini, Giuseppe Chirico, Piersandro Pallavicini, Franco Cotelli, Efrem Foglia

JBO Vol. 19 Issue 06

KEYWORDS: Diffusion, Capillaries, Luminescence, Blood, Veins, Blood circulation, Solids, Fluorescence spectroscopy, Spectroscopes, Arteries

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Proceedings Article | 1 March 2013 Paper

Structured illumination fluorescence correlation spectroscopy for velocimetry in Zebrafish embryos

Paolo Pozzi, Leone Rossetti, Laura Sironi, Stefano Freddi, Laura D'Alfonso, Michele Caccia, Margaux Bouzin, Maddalena Collini, Giuseppe Chirico

Proc. SPIE. 8580, Dynamics and Fluctuations in Biomedical Photonics X

KEYWORDS: Gold, Veins, Blood, Luminescence, Diffusion, Solids, Fluorescence correlation spectroscopy, Signal detection, Rhodamine, Nanorods

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