Live subcutaneous tumor grown in nude mouse is studied in situ with hyperspectral autofluorescence imaging and Raman spectroscopy. The purpose of the study is to develop methods for characterization of biochemical changing and of histological type of tumor without labeling. The results show that there are site depending variation in the fluorescence and Raman spectra. At the spot in which calcification is in process, Raman spectra showed a strong and specific band at 957 cm-1 due to PO4 species. The autofluosescence image can prove the histological changes based on the NADH and FAD which are major fluorophores in biological tissues. The hyperspectral image is analyzed with principal component analysis and the reconstructed images successfully depicts a different between necrotic and viable part within living subcutaneous tumor.
Our Raman probe that is called as ball-lens hollow fiber Raman probe (BHRP) had been proved
possessing capability to detect the biochemical alteration within biological tissue. Whether BHRP
has high capability and sensitivity in diagnosing the biochemical changing of tissue or not, mouse's
normal rectal and anorectal prolapse (AP) were decided to be used as a model for this non invasive
method. This AP is azoxymethane and DSS-induced mouse’s anorectal prolapse. Main outcome of
BHRP will be potential for non-invasive method in tumor diagnosing. BHRP spectra obtained were
a high quality and allowed analysis of their differences between normal rectal (control group) and
AP. After spectral acquisition and comparison with corresponding images of hematoxylin/eosinstained
section observation used to make the histopathologic diagnosing, BHRP detected some
differences within the region of moiety of DNA, protein (i.e. collagen) and lipid, then following with
the alteration of symmetric P=O stretching vibration compared with the normal rectal tissue. BHRP
discriminate normal tissue and AP in the real-time.
Problem of viruses is very actual for nowadays. Some viruses, which are responsible for human of all tumors, are about
15 %. Main purposes this study, early detection virus in live cell without labeling and in the real time by Raman
spectroscopy. Micro Raman spectroscopy (mRs) is a technique that uses a Raman spectrometer to measure the spectra of microscopic samples. According to the Raman spectroscopy, it becomes possible to study the metabolites of a live
cultured cell without labeling. We used mRs to detect the virus via HEK 293 cell line-infected adenovirus. We obtained
raman specters of lives cells with viruses in 24 hours and 7 days after the infection. As the result, there is some
biochemical changing after the treatment of cell with virus. One of biochemical alteration is at 1081 cm-1. For the clarification result, we use confocal fluorescent microscopy and transmission electron microscopy (TEM).
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