An adaptive optics optical coherence tomography (AO-OCT) system with a non-modulated pyramid wavefront sensor (P-WFS) is presented. The P-WFS is implemented as add-on to a Shack Hartmann (SH) WFS based AO-OCT system, where the AO loop is driven by either sensor. Equivalent performance of the AO-OCT is demonstrated with the P-WFS and the SH-WFS by visualizing the mosaics of different retinal cell types in image data obtained in-vivo at the fovea of a healthy volunteer. Contrary to the SH-WFS, the pupil sampling of P-WFS is flexible and may be adjusted in order to further increase the sensitivity of the sensor.
An adaptive optics optical coherence tomography (AO-OCT) system with a 3-sided pyramid wavefront sensor (P-WFS) is presented. Compared to the Shack-Hartmann WFS, the P-WFS promises better sensitivity in low-light scenarios and greater flexibility in pupil sampling. Key feature of the presented set-up is that part of the imaging light is used to illuminate the WFS. The double pass enables closed loop correction without beam modulation and speckle patterns in the sensor read-out are averaged out during scanning. The developed instrument is demonstrated with retinal images obtained in-vivo, where the cone mosaic is clearly visualized at ~4° eccentricity from the fovea.
Adaptive optics (AO) systems for retinal imaging have a small field of view (FoV) of 1°-2°. To increase the FoV we developed a multi-conjugated AO-system. By choosing different scanning patterns, aberrations introduced by the eye at different locations within the FoV can be measured by a Shack Hartmann wavefront sensor. A second deformable mirror that is conjugated to a plane anterior to the retina corrects for varying aberrations across the field. Retinal imaging using spectral-domain optical coherence tomography is performed with 4º x 4º FoV and imaging data including the visualization of the cone mosaic is presented.
The aim of this research was to visualize and measure the human gingival sulcus in vivo using the swept-source optical coherence tomography system based on 1310 nm wavelength source with the developed classification algorithm of gingival sulcus. Apart from the algorithm based examination procedure, the OCT cross-sectional images were involved in A-scan depth profile analysis to illustrate the intensity fluctuation of teeth and the periodontal tissue structures to clarify the end point of gingival sulcus. The quantitative measurement was assessed with 1.10 ± 0.26 mm. Thus, the swept-source optical coherence tomography system could be used to perform the gingival sulcus imaging.
The conventional Fourier domain optical coherence tomography system requires single scanner for two dimensional cross-sectional image and two scanners for volumetric image. Parallel spectral domain optical coherence tomography has advantage of single scanner for volumetric image, while two dimensional cross-sectional images are obtained by parallel acquisition of illuminated line on sample using area camera. In this study, the industrial inspection of optical thin film on touch screen panels was demonstrated using parallel spectral domain optical coherence tomography. The cross-sectional and volumetric images were acquired to detect the internal sub layer defects in optical thin film which are difficult to observe using visual or machine vision based inspection methods. The results indicate the possible application of the proposed system in touch screen panels inspection for quality assurance of product at consumer end.
KEYWORDS: Optical coherence tomography, In vivo imaging, Sodium, Microscopes, Scanning electron microscopy, Magnetic resonance imaging, Real time imaging, Image resolution, Agriculture, Imaging systems
In this study, Optical coherence tomography (OCT) is demonstrated as a plausible optical tool for in vivo detection of plant seeds and its morphological changes during growth. The experiment was carried out on Capsicum annuum seeds that were treated with different molar concentrations of NaCl to investigate the most optimal concentration for the seed growth. The monitoring process was carried out for 9 consecutive days. The in vivo 2D OCT images of the treated seeds were obtained and compared with seeds that were grown with sterile distilled water. The obtained results confirm the feasibility of using OCT for the proposed application. Normalized A-scan analysis method is utilized for supporting the concluded results.
Here we describe the possible application of optical coherence tomography (OCT) to inspect Marssonina coronaria infected apple blotch disease of in situ apple leaves. To fulfill the in situ field inspection requirement, we developed a compact wearable OCT system. For the confirmation of OCT results, simultaneous experiment was performed in realtime using loop-mediated isothermal amplification (LAMP), which is frequently used in agriculture. LAMP method was developed as an alternative approach for the inspection of disease. We performed field inspection for 30 consecutive days, and all the acquired results from both OCT and lamp were compared to confirm the correlation. A clear identification between healthy specimens, apparently healthy but infected specimens, and infected specimens could be obtained through the real-time OCT images, and the correlation between OCT and lamp results was confirmed through the obtained realtime lamp results. Based on this feasibility study, we conclude that the combination of both these diagnosing modalities can be effective for various novel agricultural discoveries.
A dual illumination system is proposed for cornea and retina imaging using spectral domain optical coherence tomography (SD-OCT). The system is designed to acquire cornea and retina imaging with dual illumination with limited optics and using a single spectrometer. The beam propagation for cornea and retina imaging in dual illumination enables to acquire the images of different segments. This approach will reduce the imaging time for separate corneal and retinal imaging. The in vivo imaging of both the cornea and retina of a health volunteer shows the feasibility of the system for clinical applications
A method for depth enhancement is presented using a bidirectional imaging modality for spectral domain optical coherence tomography (SD-OCT). Two precisely aligned sample arms along with two reference arms were utilized in the optical configuration to scan the samples. Using exemplary images of the optical resolution target, Scotch tape, a silicon sheet with two needles, and a leaf, we demonstrated how the developed bidirectional SD-OCT imaging method increases the ability to characterize depth-enhanced images. The results of the developed system were validated by comparing the images with the standard OCT configuration (single-sample arm setup). Given the advantages of higher resolution and the ability to visualize deep morphological structures, this method can be utilized to increase the depth dependent fall-off in samples with limited thickness. Thus, the proposed bidirectional imaging modality is apt for cross-sectional imaging of entire samples, which has the potential capability to improve the diagnostic ability.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.