There was a previous research that proposed the structured illumination confocal scanning microscope (SICSM) so as
to improve the lateral resolution of the confocal microscope. However, the image acquisition speed of the SICSM was
very slow and also an alignment error due to the mechanical rotation of a grating and a slit can easily occur. As a
theoretical study, in this paper we propose a new SI method, the cross SI method, which improves lateral resolution and
image acquisition speed. Performances of the conventional SI and the proposed SI methods are compared by analysis of
the modulation transfer function. The proposed SI method shows similar lateral resolution and can shorten the image
acquisition time compared to the conventional SI method. The cross structured illumination confocal microscope
(CSICM) is combined with the cross SI pattern optics and the line scanning confocal microscope. We have introduced a
2-D diffractive grating in order to create the cross SI pattern. The effects of the cross SI pattern, intensity and visibility,
on the system performance are analyzed. The CSICM has double the lateral resolution of the conventional microscope,
an optical sectioning ability and a fast image acquisition speed.
In-vivo confocal microscope technology can be applied to the medical imaging diagnosis and new drug development.
We present an in-vivo confocal microscope that can acquire a reflection image and a fluorescence image simultaneously
and independently. To obtain reflection confocal images, we used a linearly polarized diode laser with the wavelength of
830 nm. To acquire fluorescence confocal images, we used two diode lasers with the wavelength of 488 nm and 660 nm,
respectively. Because of a broad wavelength bandwidth from visible (488 nm) to near-IR (830 nm), we designed and
optimized the optical system to reduce various optical aberrations. With the developed in-vivo confocal microscope, we
performed ex-vivo cell imaging and in-vivo imaging of the human skin.
Recently, optical imaging system is widely used in medical purpose. By using optical imaging system specific diseases
can be easily diagnosed at early stage because optical imaging system has high resolution performance and various
imaging method. These methods are used to get high resolution image of human body and can be used to verify whether
the cell is infected by virus. Confocal microscope is one of the famous imaging systems which is used for in-vivo
imaging. Because most of diseases are accompanied with cellular level changes, doctors can diagnosis at early stage by
observing the cellular image of human organ. Current research is focused in the development of endo-microscope that
has great advantage in accessibility to human body. In this research, I designed small probe that is connected to confocal
microscope through optical fiber bundle and work as endo-microscope. And this small probe is mainly designed to
correct chromatic aberration to use various laser sources for both fluorescence type and reflection type confocal images.
By using two kinds of laser sources at the same time we demonstrated multi-modality confocal endo-microscope.
The advantage of confocal fluorescence microscopy is the ability to acquire high resolution images of fluorescent
specimens non-invasively. On the other hand, the advantage of Raman microscopy is the ability to provide the chemical
characteristics of specimens from spectroscopy. To obtain simultaneously high resolution images and chemical
characteristics of specimens, fluorescence signals from stained cell and Raman spectrum from cell itself should be
separated. By separating two kinds of signals, confocal fluorescence image and Raman spectrum are acquired
simultaneously at the same position. In this paper, we demonstrate a confocal fluorescence microscopy combined with
the Raman microscopy. And we propose a method that eliminates Raman spectrum of fluorophore itself from Raman
spectrum of the stained cell and that obtains simultaneously confocal fluorescence image from stained cell and Raman
spectrum of cell itself without replacing a cell.