Long period fiber grating (LPFG) has been actively researched in bio-sensing applications owing to its ability to sense refractive index (RI) of the surrounding medium. We investigate on the adequacy of the present state of the art to quantify adsorption of bio-molecules on the surface of the fiber confined within few tens of nanometers and possible improvements in the design of sensors suitable for bio-sensing applications.
For detecting bio-molecular interaction using long period grating (LPG) we believe that a quantitative data concerning sensitivity for addition of layers on the surface and subsequently to optimize the same appears to be more usefull than defining LPG sensitivity for a surrounding refractive index change in bulk form. For the first time, to the best of our knowledge, we quantify the shift of resonant wavelength (Δλres) of the mode of interest around the transition point as a function of unit bi-layer thickness (Δd) of poly-electrolyte, deposited by ionic self assembly, and subsequently optimize the sensitivity Δλres/Δd. Experimental result show that a shift of ~12.5 nm/bi-layer is possible with optimum number of bi-layer deposition.
Long period fiber gratings (LPFGs) have been proposed as label-free optical biosensor for a few years. Refractive index changes, which modify the fiber transmission spectrum, are still used for evaluating a biochemical interaction that occurs along the grating region. A turn-around point (TAP) LPFG was manufactured for enhancing the refractive index sensitivity of these devices. Considering the simplicity and the fast process with respect to the silanization procedure, the functionalization of the fiber was carried out by Eudragit L100 copolymer. An IgG/anti-IgG immunoassay was implemented for studying the antigen/antibody interaction. A limit of detection lower than 100 μg L-1 was achieved. Based on the same model assay, we compared the resonance wavelength shifts during the injection of 10 mg L-1 anti-IgG antigen between the TAP LPFG and a standard non-TAP one, in which the coupling occurs with a lower order cladding mode, as performance improvement of the LPFG-based biosensors.
We investigate the phase matching conditions and sensitivities of higher order metal jacketed long period gratings
(LPGs). These higher order modes have been previously demonstrated to have flatter, and therefore more sensitive,
phase matching conditions leading up to the phase matching turning point. We demonstrate this increased sensitivity as
applied to a Pd jacketed LPG hydrogen sensor illustrating an improvement in both the refractive index and temperature
sensitivity (of the 17th order mode) of an order of magnitude over the lower order (1st-9th) modes.