KEYWORDS: Red blood cells, Oxygenation, Microscopy, Tissues, Absorption, Third harmonic generation, Sum frequency generation, Spatial resolution, Resonance enhancement, Multiphoton microscopy
We present color third-order sum-frequency generation (color TSFG) microscopy, a multiphoton imaging strategy based on the simultaneous detection of several third-order coherent signals produced by two synchronized femtosecond pulse trains. We demonstrate that it can be used to obtain red blood cell (RBC)-specific label-free contrast in live zebrafish and is a promising tool for probing RBC oxygenation.
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