This work presents a compact low-cost and straightforward shadow imaging microscopy technique based on spatially resolved nano-illumination instead of spatially resolved detection. Independently addressable nano-LEDs on a regular 2D array provide the resolution of the microscope by illuminating the sample in contact with the LED array and creating a shadow image in a photodetector located on the opposite side. The microscope prototype presented here is composed by a GaN chip with an 8x8 array of 5μm-LEDs with 10 μm pitch light sources and a commercial CMOS image sensor with integrated lens used as a light collector. We describe the microscope prototype and analyze the effect of the sensing area size on image reconstruction.
This work presents a first prototype for a new approach to microscopy: a system basing its resolving power on the light emitters instead of the sensors, without using lenses. This new approach builds on the possibility of making LEDs smaller than current technology sensors, offering a new approach to microscopy we plan on developing towards superresolution. The microscope consists on a SPAD based camera, a 8x8 LED array with 5x5 μm LEDs distributed with a pitch of 10 μm, and discrete driving electronics to control them. We present simulations of the system, as well as the first microscope prototype implementing the method, and the results obtained through it.